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Recombinant Cynomolgus CD3D & CD3E, His-Fc & Flag-Fc tagged

Cat.No.: CD3E-306C
Product Overview: Recombinant Cynomolgus CD3D & CD3E (Met1-Asp117 & Met1-Ala105), fused with A DNA sequence encoding the Cynomolgus (Macaca fascicularis) CD3E extracellular domain (Met 1-Asp 117) was fused with the C-terminal his-tagged Fc region of human IgG1 at the C-ter
Source: Human Cells
Species: Cynomolgus
Tag: His-Fc & Flag-Fc
Predicted N Terminal: Gln 22 & Phe 22
Form: Lyophilized from sterile PBS, pH 7.4Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
Molecular Mass: The recombinant heterodimer of cynomolgus CD3E&CD3D comprises 679 (346+333) amino acids and has a calculated molecular mass of 76.7 (39.1+ 37.6) KDa. The apparent molecular mass of cyno CD3E&CD3D heterodimer is approximately 52 & 43 KDa respectively in SD
Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
Purity: (50.3+48.5) % as determined by SDS-PAGE
Stability: Samples are stable for up to twelve months from date of receipt at -70oC.
Storage: Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4oC before opening to recover the entire contents.

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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