Active Recombinant Human IL1B
Cat.No. : | IL1B-9908H |
Product Overview : | Recombinant Human IL1B was expressed in CHO Cells. |
- Specification
- Gene Information
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Description : | Interleukin 1 beta is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1 alpha and IL-1 beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1 beta is a secreted cytokine, IL-1 alpha is predominantly a cell-associated cytokine. |
Source : | CHO Cells |
Species : | Human |
Form : | Lyophilized after extensive dialysis against PBS. |
Bio-activity : | ED50< 10 pg/ml, measured in a cell proliferation assay using d10s cells, corresponding to a specific activity of>1 x 108 units/mg. |
Molecular Mass : | 17kDa, observed by non-reducing SDS-PAGE. |
AA Sequence : | Ala117-Ser269. |
Endotoxin : | <0.2 eu/μg, determined by lal>0.2> |
Purity : | >95% as analyzed by SDS-PAGE and HPLC. |
Storage : | Lyophilized recombinant protein remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhIL-1β should be stable up to 1 week at 4°C or up to 2 months at -20°C. |
Reconstitution : | Reconstituted in ddH2O or PBS at 100 μg/ml. |
Gene Name : | IL1B interleukin 1, beta [ Homo sapiens ] |
Official Symbol : | IL1B |
Synonyms : | IL1B; interleukin 1, beta; interleukin-1 beta; IL 1B; IL1 BETA; IL1F2; IL-1 beta; catabolin; preinterleukin 1 beta; pro-interleukin-1-beta; IL-1; IL1-BETA; |
Gene ID : | 3553 |
mRNA Refseq : | NM_000576 |
Protein Refseq : | NP_000567 |
MIM : | 147720 |
UniProt ID : | P01584 |
Chromosome Location : | 2q14 |
Pathway : | African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Apoptosis, organism-specific biosystem; |
Function : | cytokine activity; cytokine activity; growth factor activity; interleukin-1 receptor binding; protein domain specific binding; |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionInflammasome assembly initiated by IL-1β is deciphered using techniques like co-immunoprecipitation, proximity ligation assays, and super-resolution microscopy.
IL-1β's priming of immune cells is dissected through flow cytometry, RNA sequencing, and chromatin immunoprecipitation, revealing its epigenetic and transcriptional effects.
Mass spectrometry-based phosphoproteomics deciphers IL-1β's post-translational modifications, revealing regulatory sites crucial for its activation and signaling.
Bioinformatics tools integrate multi-omics data to provide a comprehensive understanding of IL-1β's roles, revealing its intricate contributions to health and disease.
ChIP-seq and DNA methylation profiling uncover IL-1β's epigenetic impact on target genes, shedding light on its role in shaping chronic inflammatory states.
IL-1β's interactions with downstream effectors are unveiled using dual-luciferase reporter assays, surface plasmon resonance, and co-crystallization studies.
Network analysis integrates omics data to unravel IL-1β's intricate cross-talk with other cytokines, mapping out complex regulatory loops in inflammation.
IL-1β's impact on the tumor microenvironment is studied using intravital microscopy, 3D culture models, and single-cell RNA sequencing to capture dynamic cell behavior.
CRISPR-Cas9 screening identifies IL-1β's non-canonical signaling mediators through functional genomics, revealing alternative pathways in cellular responses.
Single-cell RNA sequencing unveils IL-1β's context-specific responses, exposing cell type-specific signaling and gene expression patterns.
Customer Reviews (3)
Write a reviewEffective in inflammation studies.
High specificity.
Suitable for cytokine assays.
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