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Active Recombinant Human PADI4, His-tagged

Cat.No. : PADI4-380H
Product Overview : Recombinant Human PADI4 (Ala2-Pro663) fused with an N-terminal 11 kDa fusion protein, a thrombin cleavage linker and 6­His tag at C­terminal, was expressed in E. coli.
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  • Gene Information
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Description : This gene is a member of a gene family which encodes enzymes responsible for the conversion of arginine residues to citrulline residues. This gene may play a role in granulocyte and macrophage development leading to inflammation and immune response.
Source : E. coli
Species : Human
Tag : His
Predicted N Terminal : Inconclusive result, Met predicted. Protein identity confirmed by MS analysis of tryptic fragments
Formulation : Supplied as a 0.2 µm filtered solution in Sodium Acetate, NaCl, DTT, EDTA and Glycerol.
Bio-activity : Measured by its ability to catalyze the deimination of benzoyl-arginine ethyl ester. The specific activity is >1000 pmol/min/µg, as measured under the described conditions.
Molecular Mass : Predicted Molecular Mass: 87 kDa SDS-PAGE: 75-85 kDa, reducing conditions
Endotoxin : < 1.0 EU per 1 μg of the protein by the LAL method.
Purity : >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
Storage : Avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.
Gene Name : PADI4 peptidyl arginine deiminase, type IV [ Homo sapiens (human) ]
Official Symbol : PADI4
Synonyms : PADI4; PAD; PAD4; PDI4; PDI5; PADI5; peptidyl arginine deiminase, type IV; protein-arginine deiminase type-4; HL-60 PAD; PADI-H protein; protein-arginine deiminase type IV; peptidyl arginine deiminase, type V; EC 3.5.3.15
Gene ID : 23569
mRNA Refseq : NM_012387
Protein Refseq : NP_036519
MIM : 605347
UniProt ID : Q9UM07
Chromosome Location : 1p36.13
Pathway : protein citrullination
Function : calcium ion binding; protein-arginine deiminase activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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In which tissues or cell types is PADI4 predominantly expressed? 09/25/2022

PADI4 is predominantly expressed in various tissues, including synovial tissues, immune cells, and certain epithelial cells.

How does PADI4 contribute to the post-translational modification of proteins, particularly through citrullination? 07/27/2020

PADI4 plays a crucial role in citrullination, a process that converts arginine residues to citrulline, influencing protein structure and function.

What is the primary function of peptidyl arginine deiminase 4 (PADI4) in cellular processes? 06/16/2020

Peptidyl arginine deiminase 4 (PADI4) primarily functions in cellular processes, contributing to the post-translational modification of proteins through citrullination.

What is the role of PADI4 in the regulation of immune responses and autoimmune diseases? 05/25/2020

In the regulation of immune responses, PADI4 is implicated in autoimmune diseases such as rheumatoid arthritis, where it contributes to autoantibody generation.

How is dysregulation or abnormal expression of PADI4 implicated in various diseases, including rheumatoid arthritis? 12/31/2019

Dysregulation or abnormal expression of PADI4 is associated with autoimmune disorders, particularly rheumatoid arthritis.

Are there therapeutic implications or potential targets related to PADI4 in the context of autoimmune disorders? 08/26/2018

There are potential therapeutic implications related to targeting PADI4 in autoimmune disorders, making it a focus for drug development.

Are there specific substrates of PADI4, and what cellular processes are affected by its citrullination activity? 07/14/2016

Specific substrates of PADI4 include histones and other proteins, impacting cellular processes like gene regulation and signaling pathways.

Customer Reviews (3)

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Reviews
12/05/2020

    The minimal variation across different batches enhanced the repeatability of our results.

    06/14/2018

      The product's role in promoting collaboration and teamwork positively impacted the quality of our research.

      01/23/2017

        The product's compatibility with various instruments and techniques promoted teamwork in our interdisciplinary projects.

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