| Description : |
Interleukin-7 (IL-7) is a member of the interleukin family which regulates the development and survival of lymphoid and myeloid cells. It has a potent effect for the stimulation of the adaptative immune response, it promotes proliferation of lymphoid progenitors (Pre-B, Pro-B and early T cells), and the effects include mobilization, maturation, and maintenance of IL-7 responsive immune cells.The biological functions of IL-7 as a key "lymphotrophin" in JAK-STAT pathways, PI3 kinase pathway, and Src kinase pathway. IL-7 has also been shown to play a vital role in energy metabolism of T cells and regulating glucose uptake in T lymphocytes through Glut1 via activation of STAT5 and Akt. It has a thymic antiapoptotic effect, and induce the expression of antiapoptotic proteins Bcl-2 and Mcl-1 and the inhibition of preapoptotic proteins Bax and Bad. IL-7 has potential therapeutic applications for the lymphoid reconstitution of immuno-depressed patients (chemotherapy, radiation, AIDS, and bone marrow transplant). |
| Source : |
Nicotiana benthamiana |
| Species : |
Human |
| Tag : |
His |
| Form : |
Recombinant human IL-7 is lyophilized from 10mM PBS buffer pH 7.5 and 0.2 M NaCl. |
| Bio-activity : |
Biological Activity:The activity of recombinant Human Interleukin-7 is measured in a cell proliferation assay using PHA activated human peripheral blood lymphocytes. The ED50 for this effect is typically<10ng l in presence of goat anti-human igm (μ chain> |
| Molecular Mass : |
rHuman Interleukin 7 is a glycosilated polypeptide chain containing 160 amino acids (26 -177 of P13232 IL7_HUMAN ), fused to His tag at N-terminal. rHuman IL-7 migrate at approximatly 18–25 kDa by SDS-PAGE. |
| AA Sequence : |
HHHHHHHHDCDIEGKDGKQYESVLM VSIDQLLDSMKEIGSNCLNNEFNFF KRHICDANKEGMFLFRAARKLRQFL KMNSTGDFDLHLLKVSEGTTILLNC TGQVKGRKPAALGEAQPTKSLEENK SLEQKKLNDLCFLKRLLQEIKTCWN KILMGTKEH |
| Endotoxin : |
< 0.04 eu/μg protein (lal> |
| Purity : |
>97% by SDS-PAGE gel |
| Applications : |
Cell culture. Western Blot. For R+D purposes only. Purchaser must determine the suitability of the product for their particular use. |
| Stability : |
This lyophilized preparation is stable at 2-8oC for short term, long storage it should be kept at -20oC. |
| Storage : |
Reconstituted protein should be stored in working aliquots at –20°C. Repeated freezing and thawing is not recommended. |
| Reconstitution : |
Lyophilized protein should be reconstituted in water following instructions of batch Quality Control sheet. At higher concentrations the solubility may be reduced and multimers generated. Optimal concentration should be determined for specific application. |
