Active Recombinant Mouse Nt5e, His-tagged

• Cat.No.: Nt5e-250M
• Product Overview: Recombinant Mouse Nt5e (Trp29-Lys549) fused with 6­His tag at C­terminal, was expressed in CHO Cells.

Specification

• Description: 5''-nucleotidase(5''-NT), also known as ecto-5''-nucleotidaseor CD73 (Cluster ofDifferentiation 73), is an enzymethat in humans is encoded by theNT5E gene. CD73 commonly serves to convert AMP toadenosine.
• Source: CHO Cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Trp29
• Form: Supplied as a 0.2 µm filtered solution in Tris, NaCl, CaCl2 and Glycerol.
• Bio-activity: Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The specific activity is >10,000 pmol/min/µg.
• Molecular Mass: 59 kDa
• Endotoxin: < 1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

Gene Information

• Gene Name: Nt5e 5'' nucleotidase, ecto [ Mus musculus (house mouse) ]
• Official Symbol: Nt5e
• Synonyms: Nt5e; NT; Nt5; eNT; CD73; AI447961; 2210401F01Rik; 5'' nucleotidase, ecto; 5''-nucleotidase; 5''-NT; ecto-5''-nucleotidase; EC 3.1.3.5
• Gene ID: 23959
• mRNA Refseq: NM_011851
• Protein Refseq: NP_035981
• UniProt ID: Q61503
• Chromosome Location: 9; 9 E3.2
• Pathway: Metabolism of nucleotides; Nicotinate and nicotinamide metabolism; Purine metabolism
• Function: 5''-nucleotidase activity; ferrous iron binding; hydrolase activity, acting on ester bonds