Active Recombinant Human CCL20

• Cat.No.: CCL20-296H
• Product Overview: Recombinant human MIP-3α is a non-glycosylated protein, containing 70 amino acids and a molecular weight of 8.0 kDa.

Specification

• Description: Macrophage Inflammatory Protein-3 alpha (MIP-3α), also called CCL20, is expressed in the liver, lungs, lymph nodes and peripheral blood lymphocytes. MIP-3α is strongly up regulated by inflammatory signals, and down regulated by the antiinflammatory cytokine IL-10. MIP-3α signals through the G protein-coupled receptor, CCR6, and acts as a chemoattractant to lymphocytes and dendritic cells.
• Source: E.coli
• Species: Human
• Appearance: Sterile filtered white lyophilized (freeze-dried) powder.
• Form: Recombinant human MIP-3α is lyophilized with no additives.
• Bio-activity: The activity is determined by its ability to act as a chemoattractant to human T cells and is typically in the range of 7 - 70 ng/mL.
• Molecular Mass: 8.0 kDa
• AA Sequence: ASNFDCCLGY TDRILHPKFI VGFTRQLANE GCDINAIIFH TKKKLSVCAN PKQTWVKYIV RLLSKKVKNM
• Endotoxin: Measured by kinetic LAL analysis and is typically ≤ 1 EU/μg protein.
• Purity: ≥ 97%
• Stability: Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
• Reconstitution: Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions.

Gene Information

• Gene Name: CCL20 chemokine (C-C motif) ligand 20 [ Homo sapiens (human) ]
• Official Symbol: CCL20
• Synonyms: CCL20; CKb4; LARC; ST38; MIP3A; MIP-3a; SCYA20; MIP-3-alpha; C-C motif chemokine 20; chemokine (C-C motif) ligand 20; CC chemokine LARC; beta chemokine exodus-1; small-inducible cytokine A20; macrophage inflammatory protein 3 alpha; liver and activation-regulated chemokine; small inducible cytokine subfamily A (Cys-Cys), member 20
• Gene ID: 6364
• mRNA Refseq: NM_004591
• Protein Refseq: NP_004582
• MIM: 601960
• UniProt ID: P78556
• Chromosome Location: 2q36.3
• Pathway: Chemokine receptors bind chemokines; Chemokine signaling pathway; Class A/1 (Rhodopsin-like receptors)
• Function: chemokine activity