Active Recombinant Human CDH1, Fc tagged
Cat.No. : | CDH1-274H |
Product Overview : | Recombinant Human CDH1 (P12830) (Met1-Ile707), fused with the Fc region of human IgG1 at the C-terminus, was produced in Human Cells. |
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Source : | HEK293 |
Species : | Human |
Tag : | Fc |
Form : | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast adenocarcinoma cells. When cells are added to E-Cad coated plates (5 μg/mL, 100 μL/well), > 30% will adhere specifically after 90 minutes at 37 °C. |
Molecular Mass : | The recombinant human E-Cad/Fc is a disulfide-linked homodimer. The reduced monomer comprises 791 amino acids and has a predicted molecular mass of 87.1 kDa. The apparent molecular mass of the protein is approximately 116-126 kDa in SDS-PAGE under reducing conditions due to glycosylation. |
Protein Length : | Met1-Ile707 |
Endotoxin : | < 1.0 EU per μg protein as determined by the LAL method. |
Purity : | > 90 % as determined by SDS-PAGE |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Publication : |
A mechanically active heterotypic E-cadherin/N-cadherin adhesion enables fibroblasts to drive cancer cell invasion (2017)
Cholesterol metabolism and statin effects on an FH class II LDL-receptor mutation (2018)
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Gene Name : | CDH1 cadherin 1, type 1, E-cadherin (epithelial) [ Homo sapiens ] |
Official Symbol : | CDH1 |
Synonyms : | CDH1; cadherin 1, type 1, E-cadherin (epithelial); UVO; cadherin-1; CD324; E Cadherin; uvomorulin; CAM 120/80; E-Cadherin; cell-CAM 120/80; epithelial cadherin; cadherin 1, E-cadherin (epithelial); calcium-dependent adhesion protein, epithelial; CDHE; ECAD; LCAM; Arc-1; |
Gene ID : | 999 |
mRNA Refseq : | NM_004360 |
Protein Refseq : | NP_004351 |
MIM : | 192090 |
UniProt ID : | P12830 |
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◆ Lysates | ||
CDH1-938HCL | Recombinant Human CDH1 cell lysate | +Inquiry |
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CDH1-736RCL | Recombinant Rat CDH1 cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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