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Active Recombinant Canine EFNB2 protein(Met1-Ala229), hFc-tagged

Cat.No. : EFNB2-297C
Product Overview : Recombinant Canine EFNB2 (B0LDS6) (Met1-Ala229) was expressed in HEK293 with the Fc region of Human IgG1 at the C-terminus.
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Source : HEK293
Species : Canine
Tag : C-hFc
Protein length : Met1-Ala229
Form : Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity : 1. Measured by its binding ability in a functional ELISA. 2. Immobilized human EphB4-His at 10μg/mL (100μL/well) can bind Canine EFNB2-Fc3.The EC50 of Canine EFNB2-Fc3 is 25.8-60.2 ng/mL.
Molecular Mass : The recombinant Canine EFNB2/Fc is a disulfide-linked homodimer. The reduced monomer comprises 443 amino acids and has a predicted molecular mass of 49.2 kDa. The apparent molecular mass of the protein is approximately 58 and 35 kDa in SDS-PAGE under reducing conditions.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 95 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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What effect does EphB6 overexpression have on EFNB2? 05/06/2022

EphB6 overexpression up-regulates the expression of its ligand EFNB2.

What diseases is EFNB2 involved in? 01/02/2022

EFNB2 is associated with breast, colon, bladder, cervical and other cancers.

What is the biological function of EFNB2? 05/05/2021

EFNB2 is involved in tissue plasticity, tumorigenesis, and other biological processes.

Where is EFNB2 mainly expressed? 04/10/2021

EFNB2 is widely expressed in tumor vasculature.

What is the association between EFNB2 and BUC cells? 03/12/2021

EFNB2 promotes BUC cell proliferation by inhibiting apoptosis in BUC cells.

How is EphB6 related to EFNB2? 07/09/2019

EFNB2 is one of the physiological ligands of EphB6 that functions by binding to EphB6.

Customer Reviews (3)

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Reviews
01/05/2022

    During the experiment, the technician helped me solve the problems in a timely and accurate manner.

    01/02/2022

      The EFNB2 proteins purchased from your company is suitable for WB.

      01/23/2021

        Received high quality protein products and obtained satisfactory experimental results.

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