Recombinant Human LIF (P15018) (Met1-Phe202), fused with a C-terminal polyhistidine tag, was produced in Human Cells.
Predicted N Terminal:
Lyophilized from sterile PBS, pH 7.4.Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
The recombinant human LIF comprises 191 amino acids and has a predicted molecular mass of 21.2 kDa. The apparent molecular mass of the protein is approximately 35-42 kDa in SDS-PAGE under reducing conditions.
< 1.0 eu per μg of the protein as determined by the LAL method.
>95 % as determined by SDS-PAGE
Samples are stable for up to twelve months from date of receipt at -70oC.
Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4oC before opening to recover the entire contents.