Active Recombinant Human MMP14
Cat.No. : | MMP14-808H |
Product Overview : | Active Matrix Metalloproteinase-14 (MMP-14, Membrane-Type Matrix Metalloproteinase 1, MT1-MMP) catalytic domain from human cDNA. The enzyme consists of the catalytic domain of human MMP-14 (Tyr112-Arg298, NM_004995) with a C-terminal purification tag. This represents a naturally-occurring active form of MMP-14 which lacks the C-terminal hemopexin domain. MMPs lacking this domain cannot cleave native collagens; however, activity toward other targets such as gelatin, casein, or peptide substrates is unaffected. |
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Description : | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP''s are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion. |
Source : | E. coli |
Species : | Human |
Form : | Liquid. In 50mM TRIS, pH 7.5, containing 5mM calcium chloride, 300mM sodium chloride, 20μM zinc chloride, 0.5% Brij-35, and 30% glycerol. |
Bio-activity : | 73 U/µg. One U=100 pmol/min at 37°C using the colorimetric thiopeptolide Ac-Pro-Leu-Gly-S-Leu-Leu-Gly-OEt as substrate. Preincubation of MMP-14 catalytic domain at 13.6nM with the broad spectrum inhibitor GM6001 at 5nM for 1 hour inhibits enzymatic activity by 93%. |
Molecular Mass : | 22.5 kDa |
Applications : | Study enzyme kinetics, cleave target substrates, and screen for inhibitors. |
Stability : | NOTE: When stored at -70°C, this enzyme is stable at the concentration supplied, in its current storage buffer. Procedures such as dilution of the enzyme followed by refreezing could lead to loss of activity. |
Storage : | -80°C |
Gene Name : | MMP14 matrix metallopeptidase 14 (membrane-inserted) [ Homo sapiens(human) ] |
Official Symbol : | MMP14 |
Synonyms : | MMP14; 1; MMP-14; MMP-X1; MT-MMP; MT1MMP; MTMMP1; MT1-MMP; MT-MMP 1; matrix metallopeptidase 14 (membrane-inserted); matrix metalloproteinase-14; membrane type 1 metalloprotease; membrane-type matrix metalloproteinase 1; membrane-type-1 matrix metalloproteinase; EC 3.4.24.80 |
Gene ID : | 4323 |
mRNA Refseq : | NM_004995 |
Protein Refseq : | NP_004986 |
MIM : | 600754 |
UniProt ID : | P50281 |
Chromosome Location : | 14q11-q12 |
Pathway : | AGE/RAGE pathway; Degradation of collagen; Extracellular matrix organization |
Function : | calcium ion binding; metalloendopeptidase activity; sequence-specific DNA binding transcription factor activity |
Products Types
◆ Recombinant Protein | ||
MMP14-181H | Recombinant Human MMP14 Protein, His-tagged | +Inquiry |
Mmp14-4098M | Recombinant Mouse Mmp14 Protein, Myc/DDK-tagged | +Inquiry |
MMP14-571H | Recombinant Human MMP14 Protein, MYC/DDK-tagged | +Inquiry |
MMP14-1418H | Recombinant Human MMP14 Protein, His (Fc)-Avi-tagged | +Inquiry |
MMP14-5599M | Recombinant Mouse MMP14 Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
MMP14-4279HCL | Recombinant Human MMP14 293 Cell Lysate | +Inquiry |
◆ Assay kits | ||
Kit-2123 | MMP-14 Inhibitor Screening Kit | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionMMP14, also known as matrix metalloproteinase 14, is primarily expressed by cells of mesenchymal origin, such as fibroblasts and endothelial cells. Its expression is tightly regulated at multiple levels, including transcriptional, post-transcriptional, and post-translational mechanisms. Transcriptional regulation involves the binding of various transcription factors, such as AP-1 and SP-1, to the MMP14 promoter region. Additionally, microRNAs and RNA-binding proteins can modulate its mRNA stability and translation.
MMP14 plays a crucial role in cancer progression and metastasis. It facilitates tumor invasion by degrading components of the extracellular matrix, promoting cancer cell migration, and aiding in angiogenesis. MMP14 also influences tumor cell behavior by interacting with other proteins involved in cell signaling pathways and epithelial-mesenchymal transition. Its overexpression is associated with poor prognosis in various types of cancer.
Overexpression of MMP14 has been strongly associated with tumor progression and poor prognosis in various types of cancer. Increased levels of MMP14 lead to enhanced degradation of the ECM, promoting tumor cell invasion and metastasis. Additionally, MMP14 can activate other matrix metalloproteinases (MMPs), further amplifying the proteolytic activity and facilitating tumor growth and angiogenesis. Moreover, MMP14 has been implicated in tumor-associated angiogenesis and immunosuppression, contributing to tumor progression and metastasis.
Targeting MMP14 holds potential for therapeutic interventions in cancer and other diseases. Strategies such as selective inhibition of MMP14 activity, antibody-based therapies, and gene silencing techniques are being explored. However, the development of MMP14-targeted therapies faces challenges, including the need for specificity, avoiding off-target effects, and optimizing delivery methods to reach the tumor microenvironment.
Yes, there are endogenous inhibitors of MMP14, known as tissue inhibitors of metalloproteinases (TIMPs). TIMPs bind to the active site of MMP14 and prevent its activity. TIMP-2 is a specific inhibitor of MMP14 and forms a complex with MMP14, regulating its enzymatic activity. The balance between MMP14 and TIMPs is crucial for maintaining tissue homeostasis and preventing excessive extracellular matrix degradation. Dysregulation of this balance can contribute to pathological conditions, including cancer progression.
MMP14 plays a crucial role in tissue remodeling processes, such as embryogenesis, wound healing, and tissue repair. It is essential for the degradation and turnover of extracellular matrix components during tissue restructuring. MMP14 also influences cell behavior and migration during tissue development and regeneration. Additionally, it participates in the modulation of immune responses and angiogenesis, which are crucial for proper tissue remodeling.
MMP14 possesses a catalytic domain responsible for substrate cleavage. It predominantly cleaves components of the extracellular matrix, such as collagen type I and type II. The interaction between MMP14 and its substrates occurs through specific binding sites within the substrate proteins. This interaction is facilitated by the hemopexin-like domain of MMP14, which acts as a docking site for substrate recognition and binding.
Customer Reviews (3)
Write a reviewThe versatility of this reagent is evident in its capacity to cater to a plethora of sample types, offering a broad array of potential applications.
Backed by its user-friendly nature, this protein reagent saves me considerable time and effort, making it highly suitable for fast-paced laboratory work.
Brimming with enchantment, this experimental reagent illuminates your scientific odyssey.
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