| Description : |
Transforming growth factor beta 2 (TGFB2) is also known as TGF-β2, TGF-beta2, Glioblastoma-derived T-cell suppressor factor, G-TSF, BSC-1 cell growth inhibitor, Polyergin, Cetermin, and is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development. Mature human TGF-β2 shows 100% aa identity with porcine, canine, equine and bovine TGF-β2, and 97% aa identity with mouse and rat TGF-β2. It demonstrates crossspecies activity. It is an extracellular glycosylated protein. It is known to suppress the effects of interleukin dependent T-cell tumors. The increased levels of TGF beta 2 triggered a neuronal cell death pathway related to Alzheimer"s disease (AD) by binding to the extracellular domain of amyloid beta precursor protein (APP). The upregulation of the TGF beta 2 level is a common pathological feature of AD brains and suggests that it may be closely linked to the development of neuronal death related to AD. TGF-beta regulates key mechanisms of tumor development, namely immunosuppression, metastasis, angiogenesis, and proliferation. TGF beta 2 suppression is a promising therapeutic approach for malignant tumor therapy. |
| Source : |
HEK293 |
| Species : |
Human |
| Form : |
Lyophilized from 0.22 μm filtered solution in TFA, acetonitrile. Normally Mannitol or Trehalose are added as protectants before lyophilization. |
| Bio-activity : |
The bio-activity was determined by its ability to inhibit IL-4 induced HT-2 cell proliferation. The ED50<0.1 ng/ml, corresponding to a specific activity of>1X10^7 Unit/mg. |
| Molecular Mass : |
rhTGFB2 contains no "tags" and has a calculated MW of 12.7 kDa (monomer). DTT-reduced protein migrates as a 13 kDa polypeptide and the non-reduced cystine-linked homodimer migrates as a 25 kDa protein. |
| Endotoxin : |
Less than 1.0 EU per μg of the rhTGFB2 by the LAL method.. |
| Purity : |
>98% as determined by SDS-PAGE of reduced (+) and non-reduced (-) rhTGFB2. |
| Storage : |
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4 centigrade-8 centigrade); After reconstitution under sterile conditions for 1 month (4 centigrade-8 centigrade) or 3 months (-20 centigrade to -70 centigrade). |
