Recombinant streptavidin

• Cat.No.: Streptavidin-501
• Product Overview: Recombinant streptavidin from Streptomyces avidinii, was produced in Escherichia coli.

Specification

• Description: Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a KD of ~1×10^-15M. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogeneity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native streptavidin.
• Source: E. coli
• Form: Lyophilized in 10 mM potassium phosphate buffer pH 6.5
• Molecular Mass: ~53,000 per tetramer.
• AA Sequence: MAEAGITGTW YNQLGSTFIV TAGADGALTG TYESAVGNAE SRYVLTGRYD SAPATDGSGT ALGWTVAWKN NYRNAHSATT WSGQYVGGAE ARINTQWLLT SGTTEANAWK STLVGHDTFT KVKPSAAS
• Endotoxin: Less than 1EU/μg of rStreptavidin as determined by LAL method.
• Purity: >95% by SDS-PAGE
• Unit Definition: >17 U/mg (one unit binds 1 μg D-biotin at pH 8.9)
• Usage: This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
• Stability: 2 years after shipping
• Storage: -20 centigrade. Avoid repeated freeze/thaw cycles.
• Reconstitution: Dissolve with double distilled water
• Shipping: Room temperature
• Publication:
  Se-SAD serial femtosecond crystallography datasets from selenobiotinyl-streptavidin. (2017)
  Selenium single-wavelength anomalous diffraction de novo phasing using an X-ray-free electron laser. (2016)
  Cooperative Allostery and Structural Dynamics of Streptavidin at Cryogenic- and Ambient-temperature (2021)
  A Brief Tour of the Apo-Streptavidin Structures at the SSRL and LCLS Facilities (2021)