Recombinant Human MANF protein
Cat.No. : | MANF-536H |
Product Overview : | Recombinant Human MANF protein was expressed in Escherichia coli. |
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Description : | MANF is a secreted neurotrophic factor that is expressed in brain, neuronal and certain non-neuronal tissues. It has been shown to promote survival, growth and function of dopamine specific neurons. MANF and its structural homolog CDNF, each contain an N-terminal saposin-like lipid binding domain, and a carboxyl-terminal domain, which is not homologous to previously characterized protein structures. MANF and CDNF can prevent 6-OHDA induced degeneration of dopaminergic neurons by triggering survival pathways in a rat experimental model of Parkinson disease. Mature human MANF is 99 %, 98 % and 96 % a.a. identical to mature rat, mouse and bovine MANF respectively. |
Source : | E.coli |
Species : | Human |
Form : | Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4. |
Bio-activity : | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 20 μg/ml, corresponding to a specific activity of > 50 IU/mg. |
Molecular Mass : | Approximately 18.2 kDa, a single non-glycosylated polypeptide chain containing158 amino acids. |
Protein length : | 158 |
AA Sequence : | LRPGDCEVCISYLGRFYQDLKDRDV TFSPATIENELIKFCREARGKENRL CYYIGATDDAATKIINEVSKPLAHH IPVEKICEKLKKKDSQICELKYDKQ IDLSTVDLKKLRVKELKKILDDWGE TCKGCAEKSDYIRKINELMPKYAPK AASARTDL |
Endotoxin : | Less than 1 EU/µg of rHuMANF as determined by LAL method. |
Purity : | >95% by SDS-PAGE and HPLC analysis. |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
Reconstitution : | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
Publication : |
MANF protects pancreatic acinar cells against alcohol-induced endoplasmic reticulum stress and cellular injury (2021)
MANF inhibits Sjo ̈gren’s syndrome salivary gland epithelial cell apoptosis and antigen expression of Ro52/SSA through endoplasmic reticulum stress/ autophagy pathway (2023)
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Gene Name : | MANF |
Official Symbol : | MANF |
Synonyms : | MANF; mesencephalic astrocyte-derived neurotrophic factor; arginine rich, mutated in early stage tumors , ARMET; ARP; arginine-rich, mutated in early stage tumors; ARMET; MGC142148; MGC142150; |
Gene ID : | 7873 |
mRNA Refseq : | NM_006010 |
Protein Refseq : | NP_006001 |
MIM : | 601916 |
UniProt ID : | P55145 |
Products Types
◆ Recombinant Protein | ||
MANF-702H | Active Recombinant Human MANF Protein | +Inquiry |
Manf-1722M | Recombinant Mouse Manf Protein, Myc/DDK-tagged | +Inquiry |
MANF-522H | Recombinant Human MANF Protein, His-tagged | +Inquiry |
MANF-2015H | Recombinant Human MANF Protein, His-tagged | +Inquiry |
MANF-327H | Recombinant Human MANF Protein, MYC/DDK-tagged | +Inquiry |
◆ Lysates | ||
MANF-1581MCL | Recombinant Mouse MANF cell lysate | +Inquiry |
MANF-2212HCL | Recombinant Human MANF cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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