| Description : |
Recombinant Mouse Tumor Necrosis Factor-alpha (rM TNF-α) is secreted by macrophages, monocytes, neutrophils, T-cells, NK-cells following their stimulation by bacterial LPS. TNF-α causes cytolysis and cytostasis of many tumor cell lines in vitro. It also enhances phagocytosis and cytotoxicity in neutrophilic granulocytes and modulates the expression of many other proteins. Cells expressing CD4 secrete TNF-α while CD8 cells secrete little or no TNF-alpha. |
| Source : |
Streptomyces Lividans |
| Species : |
Mouse |
| Form : |
Each mg contains 50mM Tris-HCL (pH 8) and 20mM sodium chloride. |
| Bio-activity : |
rM TNF-alpha is fully biologically active when compared to a standard. The ED50 as determined by the cytolysis of mouse L929 cells in the presence of Actinomycin D is less than 0.05ng/ml. |
| Molecular Mass : |
17.3 kDa |
| AA Sequence : |
MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL |
| Endotoxin : |
Endotoxin level as measured by LAL is<0.01ng g or><0.1eu> |
| Purity : |
Purity greater than 98% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE. |
| Stability : |
Product is stable for up to 3 years from manufacturing date, when stored lyophilized at -20centigrade. Reconstituted material should be aliquoted and frozen at -20centigrade. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term stora |
| Reconstitution : |
It is recommended to reconstitute the lyophilized product with sterile water to a concentration of 0.1 mg/ml. It can be further diluted into other aqueous solutions. |
| Shipping : |
Can be shipped ambient. Product should be stored at -20centigrade upon receipt. |
