Active Recombinant Human MDM2, His-tagged

• Cat.No.: MDM2-105H
• Product Overview: Recombinant Human MDM2 was expressed in the E. coli.

Specification

• Description: This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid
• Bio-activity: 1 unit equals 1 nanogram of purified protein.
• Applications: SDS-PAGE
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
• Concentration: 20% Glycerol, 20mM Tris Cl, 100mM Potassium chloride, 1mM DTT, 0.2mM EDTA, pH 8.0
• Preservative: None

Gene Information

• Gene Name: MDM2 MDM2 oncogene, E3 ubiquitin protein ligase [ Homo sapiens(human) ]
• Official Symbol: MDM2
• Synonyms: MDM2, HDMX; hdm2; ACTFS; MDM2 oncogene, E3 ubiquitin protein ligase; E3 ubiquitin-protein ligase Mdm2; oncoprotein Mdm2; Mdm2, p53 E3 ubiquitin protein ligase homolog; double minute 2, human homolog of; p53-binding protein; Mdm2, transformed 3T3 cell double minute 2, p53 binding protein; EC 6.3.2.-
• Gene ID: 4193
• mRNA Refseq: NM_002392
• Protein Refseq: NP_002383
• MIM: 164785
• UniProt ID: Q00987
• Chromosome Location: 12q14.3-q15
• Pathway: AKT phosphorylates targets in the cytosol; Androgen receptor signaling pathway; Bladder cancer
• Function: enzyme binding; identical protein binding; peroxisome proliferator activated receptor binding