Active Recombinant Human KIT, GST-tagged
Cat.No. : | KIT-112H |
Product Overview : | Recombinant human c-KIT (V559D T670I) (544-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. |
- Specification
- Gene Information
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Description : | c-KIT is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). c-KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. c-KIT together with its ligand regulates growth and activation of a variety of hemopoietic and non-hemopoietic cells. Mutations in c-KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Recently, deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has served as a focal point in therapeutic strategies aimed at curbing neoplastic mast cell growth. |
Source : | Sf9 insect cells using baculovirus |
Species : | Human |
Tag : | GST |
Form : | Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
Bio-activity : | The specific activity of KIT was determined to be 1.8 nmol /min/mg |
Molecular Mass : | ~73 kDa |
Purity : | >70% by densitometry |
Applications : | Kinase Assay, Western Blot |
Storage : | Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. |
Concentration : | 0.05 μg/μl |
Gene Name : | KIT v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog [ Homo sapiens ] |
Official Symbol : | KIT |
Synonyms : | KIT; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; PBT, piebald trait; mast/stem cell growth factor receptor Kit; C Kit; CD117; SCFR; p145 c-kit; proto-oncogene c-Kit; piebald trait protein; soluble KIT variant 1; tyrosine-protein kinase Kit; proto-oncogene tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein; PBT; C-Kit; |
Gene ID : | 3815 |
mRNA Refseq : | NM_000222 |
Protein Refseq : | NP_000213 |
MIM : | 164920 |
UniProt ID : | P10721 |
Chromosome Location : | 4q11-q12 |
Pathway : | Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; C-MYB transcription factor network, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Endocytosis, organism-specific biosystem; Endocytosis, conserved biosystem; |
Function : | ATP binding; cytokine binding; metal ion binding; nucleotide binding; protease binding; protein binding; protein homodimerization activity; protein tyrosine kinase activity; receptor activity; receptor signaling protein tyrosine kinase activity; stem cell factor receptor activity; transmembrane receptor protein tyrosine kinase activity; |
Products Types
◆ Lysates | ||
KIT-1324RCL | Recombinant Rat KIT cell lysate | +Inquiry |
KIT-2114MCL | Recombinant Mouse KIT cell lysate | +Inquiry |
KIT-001HCL | Recombinant Human KIT cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionKIT expression varies across tissues due to tissue-specific transcription factors, epigenetic modifications, and microenvironmental cues influencing its regulation.
Transcription factors like PU.1 and GATA-2, along with epigenetic modifications, regulate KIT gene expression.
KIT is crucial for germ cell survival and proliferation; mutations may lead to infertility.
KIT maintains and influences stem cell behavior, holding potential in regenerative medicine.
KIT is vital in hematopoietic stem cell development; mutations link to leukemia and mastocytosis.
Customer Reviews (3)
Write a reviewThe quality control measures are evident in the performance of this product.
Exceptional service!
Rapid delivery! Ordered and received the product within a short timeframe, allowing us to proceed with our experiments without delays.
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