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Active Recombinant Human KIT, GST-tagged

Cat.No. : KIT-112H
Product Overview : Recombinant human c-KIT (V559D T670I) (544-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
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Description : c-KIT is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). c-KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. c-KIT together with its ligand regulates growth and activation of a variety of hemopoietic and non-hemopoietic cells. Mutations in c-KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Recently, deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has served as a focal point in therapeutic strategies aimed at curbing neoplastic mast cell growth.
Source : Sf9 insect cells using baculovirus
Species : Human
Tag : GST
Form : Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Bio-activity : The specific activity of KIT was determined to be 1.8 nmol /min/mg
Molecular Mass : ~73 kDa
Purity : >70% by densitometry
Applications : Kinase Assay, Western Blot
Storage : Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Concentration : 0.05 μg/μl
Gene Name : KIT v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog [ Homo sapiens ]
Official Symbol : KIT
Synonyms : KIT; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; PBT, piebald trait; mast/stem cell growth factor receptor Kit; C Kit; CD117; SCFR; p145 c-kit; proto-oncogene c-Kit; piebald trait protein; soluble KIT variant 1; tyrosine-protein kinase Kit; proto-oncogene tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein; PBT; C-Kit;
Gene ID : 3815
mRNA Refseq : NM_000222
Protein Refseq : NP_000213
MIM : 164920
UniProt ID : P10721
Chromosome Location : 4q11-q12
Pathway : Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; C-MYB transcription factor network, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Endocytosis, organism-specific biosystem; Endocytosis, conserved biosystem;
Function : ATP binding; cytokine binding; metal ion binding; nucleotide binding; protease binding; protein binding; protein homodimerization activity; protein tyrosine kinase activity; receptor activity; receptor signaling protein tyrosine kinase activity; stem cell factor receptor activity; transmembrane receptor protein tyrosine kinase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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How does KIT gene expression vary in different tissues, and what factors contribute to tissue-specific regulation? 02/13/2022

KIT expression varies across tissues due to tissue-specific transcription factors, epigenetic modifications, and microenvironmental cues influencing its regulation.

How is the expression of the KIT gene regulated at the transcriptional level? 01/14/2022

Transcription factors like PU.1 and GATA-2, along with epigenetic modifications, regulate KIT gene expression.

Contribution of KIT gene to germ cell maintenance in the testes and ovaries? 01/17/2020

KIT is crucial for germ cell survival and proliferation; mutations may lead to infertility.

What is the role of KIT gene in stem cell biology and regenerative medicine? 12/25/2019

KIT maintains and influences stem cell behavior, holding potential in regenerative medicine.

Role of KIT gene in hematopoiesis and implications in hematological disorders? 11/03/2018

KIT is vital in hematopoietic stem cell development; mutations link to leukemia and mastocytosis.

Customer Reviews (3)

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Reviews
11/28/2020

    The quality control measures are evident in the performance of this product.

    04/02/2019

      Exceptional service!

      07/19/2018

        Rapid delivery! Ordered and received the product within a short timeframe, allowing us to proceed with our experiments without delays.

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