Active Recombinant Human PCTK1 (CDK16)/CyclinY, GST-tagged

• Cat.No.: CDK16-115H
• Product Overview: Recombinant human PCTK1 (CDK16) (107-end) and Cyclin Y (2-end) were co-expressed by baculovirus in Sf9 insect cells using N-terminal GST tags.

Specification

• Description: PCTK1 or PCTAIRE PROTEIN KINASE 1 belongs to the cdc2/cdkx subfamily of the ser/thr family of protein kinases. PCTK1 plays a role in signal transduction cascades in terminally differentiated cells, in exocytosis, and in transport of secretory cargo from the endoplasmic reticulum. PCTK1 is ubiquitously expressed with the highest levels detected in the brain and testis. PCTK1 also contained the gene coding for ubiquitin-activating enzyme UBE1 that previously shown to mapped to Xp11.3.
• Source: Sf9 insect cells using baculovirus
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: The specific activity of PCTK1 (CDK16)/Cyclin was determined to be 40.0 nmol /min/mg
• Molecular Mass: PCTK1 (CDK16) ~ 66kDa and Cyclin Y~ 64kDa
• Purity: >90% by densitometry
• Applications: Kinase Assay, Western Blot
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μl

Gene Information

• Gene Name: CDK16 cyclin-dependent kinase 16 [ Homo sapiens ]
• Official Symbol: CDK16
• Synonyms: CDK16; cyclin-dependent kinase 16; PCTAIRE protein kinase 1 , PCTK1; FLJ16665; PCTAIRE; PCTAIRE1; PCTGAIRE; serine/threonine protein kinase; PCTAIRE-motif protein kinase 1; cell division protein kinase 16; serine/threonine-protein kinase PCTAIRE-1; PCTK1
• Gene ID: 5127
• mRNA Refseq: NM_001170460
• Protein Refseq: NP_001163931
• MIM: 311550
• UniProt ID: Q00536
• Chromosome Location: Xp11
• Function: ATP binding; cyclin-dependent protein kinase activity; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity