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Active Recombinant Human EHMT2, GST-tagged

Cat.No. : EHMT2-177H
Product Overview : Recombinant human EHMT2 (807-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
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Description : EHMT2 is a lysine-preferring histone methyltransferase. EHMT2 can methylate itself at K165 and this automethylation is necessary and sufficient to mediate in vivo interaction of EHMT2 with heterochromatin protein-1 (HP1). EHMT2 is responsible for the majority of dimethylation of histone H3 at lysine 9 (H3K9me2) and is required for the efficient repression of developmentally regulated genes during embryonic stem cell differentiation. EHMT2 is overexpressed in various cancers and can can specifically methylate p53 at Lys(373). EHMT2 polymorphisms have been associated with colorectal cancer.
Source : Sf9 insect cells using baculovirus
Species : Human
Tag : GST
Form : Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Bio-activity : The specific activity of EHMT2 was determined to be 15 nmol /min/mg
Molecular Mass : ~75 kDa
Purity : >95% by densitometry
Applications : Methyltransferase Assay
Storage : Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Concentration : 0.1 μg/μl
Gene Name : EHMT2 euchromatic histone-lysine N-methyltransferase 2 [ Homo sapiens ]
Official Symbol : EHMT2
Synonyms : EHMT2; euchromatic histone-lysine N-methyltransferase 2; BAT8, C6orf30, chromosome 6 open reading frame 30 , HLA B associated transcript 8; histone-lysine N-methyltransferase EHMT2; Em:AF134726.3; G9A; KMT1C; NG36/G9a; protein G9a; H3-K9-HMTase 3; G9A histone methyltransferase; HLA-B associated transcript 8; HLA-B-associated transcript 8; lysine N-methyltransferase 1C; ankyrin repeat-containing protein; histone H3-K9 methyltransferase 3; histone-lysine N-methyltransferase, H3 lysine-9 specific 3; BAT8; GAT8; NG36; C6orf30; FLJ35547; DKFZp686H08213;
Gene ID : 10919
mRNA Refseq : NM_006709
Protein Refseq : NP_006700
MIM : 604599
UniProt ID : Q96KQ7
Chromosome Location : 6p21.3
Pathway : Gene Expression, organism-specific biosystem; Lysine degradation, organism-specific biosystem; Lysine degradation, conserved biosystem; RNA Polymerase I Promoter Clearance, organism-specific biosystem; RNA Polymerase I Transcription, organism-specific biosystem; RNA Polymerase I Transcription Initiation, organism-specific biosystem; RNA Polymerase I, RNA Polymerase III, and Mitochondrial Transcription, organism-specific biosystem;
Function : histone methyltransferase activity (H3-K27 specific); histone methyltransferase activity (H3-K9 specific); metal ion binding; methyltransferase activity; p53 binding; protein binding; protein-lysine N-methyltransferase activity; transferase activity; zinc ion binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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What are the consequences of dysregulated EHMT2 expression in cancer cells? 03/08/2022

Aberrant EHMT2 expression is associated with cancer progression, contributing to altered gene expression patterns and epigenetic changes in cancer cells.

How is the activity of EHMT2 regulated at the molecular level? 10/31/2021

EHMT2 activity can be regulated by post-translational modifications, protein-protein interactions, and its association with cofactors and other chromatin-modifying enzymes.

Can EHMT2-mediated H3K9 methylation affect the recruitment of chromatin remodeling complexes? 12/25/2019

Yes, EHMT2-mediated H3K9 methylation can influence the recruitment of chromatin remodeling complexes, impacting the overall chromatin structure and gene expression.

What is the role of EHMT2 in X-chromosome inactivation? 09/20/2018

EHMT2 is involved in X-chromosome inactivation by contributing to the establishment of repressive chromatin marks on one of the X chromosomes in female cells.

How is the expression of the EHMT2 gene regulated in cells? 07/09/2018

The expression of EHMT2 is regulated by various factors, including cellular signaling pathways and epigenetic modifications, contributing to its dynamic role in different cellular contexts.

Customer Reviews (3)

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Reviews
10/22/2021

    The quality of this recombined protein is unmatched, providing robust and consistent results in our assays.

    02/29/2020

      Timely delivery is crucial for our lab operations, meeting our expectations.

      06/05/2019

        Excellent customer service—responsive and knowledgeable staff helped us choose the most suitable recombined protein for our experiments.

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