Active Recombinant Human EHMT2, GST-tagged
Cat.No. : | EHMT2-177H |
Product Overview : | Recombinant human EHMT2 (807-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. |
- Specification
- Gene Information
- Related Products
Description : | EHMT2 is a lysine-preferring histone methyltransferase. EHMT2 can methylate itself at K165 and this automethylation is necessary and sufficient to mediate in vivo interaction of EHMT2 with heterochromatin protein-1 (HP1). EHMT2 is responsible for the majority of dimethylation of histone H3 at lysine 9 (H3K9me2) and is required for the efficient repression of developmentally regulated genes during embryonic stem cell differentiation. EHMT2 is overexpressed in various cancers and can can specifically methylate p53 at Lys(373). EHMT2 polymorphisms have been associated with colorectal cancer. |
Source : | Sf9 insect cells using baculovirus |
Species : | Human |
Tag : | GST |
Form : | Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
Bio-activity : | The specific activity of EHMT2 was determined to be 15 nmol /min/mg |
Molecular Mass : | ~75 kDa |
Purity : | >95% by densitometry |
Applications : | Methyltransferase Assay |
Storage : | Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. |
Concentration : | 0.1 μg/μl |
Gene Name : | EHMT2 euchromatic histone-lysine N-methyltransferase 2 [ Homo sapiens ] |
Official Symbol : | EHMT2 |
Synonyms : | EHMT2; euchromatic histone-lysine N-methyltransferase 2; BAT8, C6orf30, chromosome 6 open reading frame 30 , HLA B associated transcript 8; histone-lysine N-methyltransferase EHMT2; Em:AF134726.3; G9A; KMT1C; NG36/G9a; protein G9a; H3-K9-HMTase 3; G9A histone methyltransferase; HLA-B associated transcript 8; HLA-B-associated transcript 8; lysine N-methyltransferase 1C; ankyrin repeat-containing protein; histone H3-K9 methyltransferase 3; histone-lysine N-methyltransferase, H3 lysine-9 specific 3; BAT8; GAT8; NG36; C6orf30; FLJ35547; DKFZp686H08213; |
Gene ID : | 10919 |
mRNA Refseq : | NM_006709 |
Protein Refseq : | NP_006700 |
MIM : | 604599 |
UniProt ID : | Q96KQ7 |
Chromosome Location : | 6p21.3 |
Pathway : | Gene Expression, organism-specific biosystem; Lysine degradation, organism-specific biosystem; Lysine degradation, conserved biosystem; RNA Polymerase I Promoter Clearance, organism-specific biosystem; RNA Polymerase I Transcription, organism-specific biosystem; RNA Polymerase I Transcription Initiation, organism-specific biosystem; RNA Polymerase I, RNA Polymerase III, and Mitochondrial Transcription, organism-specific biosystem; |
Function : | histone methyltransferase activity (H3-K27 specific); histone methyltransferase activity (H3-K9 specific); metal ion binding; methyltransferase activity; p53 binding; protein binding; protein-lysine N-methyltransferase activity; transferase activity; zinc ion binding; |
Products Types
◆ Recombinant Protein | ||
EHMT2-014H | Recombinant Human EHMT2 Protein, GST-tagged | +Inquiry |
EHMT2-2690M | Recombinant Mouse EHMT2 Protein, His (Fc)-Avi-tagged | +Inquiry |
EHMT2-2838H | Recombinant Human EHMT2 Protein, His (Fc)-Avi-tagged | +Inquiry |
Ehmt2-455M | Recombinant Mouse Ehmt2 Protein, MYC/DDK-tagged | +Inquiry |
EHMT2-100H | Recombinant Human EHMT2 Protein, GST-tagged | +Inquiry |
◆ Lysates | ||
EHMT2-6684HCL | Recombinant Human EHMT2 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Q&As
- Reviews
Q&As (5)
Ask a questionAberrant EHMT2 expression is associated with cancer progression, contributing to altered gene expression patterns and epigenetic changes in cancer cells.
EHMT2 activity can be regulated by post-translational modifications, protein-protein interactions, and its association with cofactors and other chromatin-modifying enzymes.
Yes, EHMT2-mediated H3K9 methylation can influence the recruitment of chromatin remodeling complexes, impacting the overall chromatin structure and gene expression.
EHMT2 is involved in X-chromosome inactivation by contributing to the establishment of repressive chromatin marks on one of the X chromosomes in female cells.
The expression of EHMT2 is regulated by various factors, including cellular signaling pathways and epigenetic modifications, contributing to its dynamic role in different cellular contexts.
Customer Reviews (3)
Write a reviewThe quality of this recombined protein is unmatched, providing robust and consistent results in our assays.
Timely delivery is crucial for our lab operations, meeting our expectations.
Excellent customer service—responsive and knowledgeable staff helped us choose the most suitable recombined protein for our experiments.
Ask a Question for All EHMT2 Products
Required fields are marked with *
My Review for All EHMT2 Products
Required fields are marked with *
Inquiry Basket