Recombinant Human UBE2K protein

• Cat.No.: UBE2K-208H
• Product Overview: Recombinant Human UBE2K protein was expressed in Escherichia coli.

Specification

• Description: Ubiquitin-conjugating enzyme E2 K belongs to the ubiquitin-conjugating enzyme family and is encoded by the UBE2K gene in humans. The ubiquitin-conjugating enzymes, also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes, take part in the second step in the ubiquitination reaction. In this reaction, E1 activates the ubiquitin by covalently attaching the molecule to its active site cysteine residue. The activated ubiquitin is then transferred to an E2 cysteine and then the E2 molecule binds E3 via a structurally conserved binding region. The ubiquitination reaction can modify proteins and regulate protein degradation. The UBE2K has been shown to interact with Huntingtin and RNF2. Additionally, it has been implicated in the degradation of huntingtin and suppression of apoptosis.
• Source: E.coli
• Species: Human
• Form: 0.2μm Filtered concentrated solution in 50 mM HEPES, pH 8.0, with 100 mM NaCl, 10 % Glycerol, 5 % Trehalose, 1 mM DTT.
• Molecular Mass: Approximately 23.4 kDa, a single non-glycosylated polypeptide chain containing 200 amino acids (a.a.) of human UBE2K and 8 a.a. vector sequence including 6 × His tag at N-terminus.
• Protein length: 208
• AA Sequence: MHHHHHHAMANIAVQRIKREFKEVL KSEETSKNQIKVDLVDENFTELRGE IAGPPDTPYEGGRYQLEIKIPETYP FNPPKVRFITKIWHPNISSVTGAIC LDILKDQWAAAMTLRTVLLSLQALL AAAEPDDPQDAVVANQYKQNPEMFK QTARLWAHVYAGAPVSSPEYTKKIE NLCAMGFDRNAVIVALSSKSWDVET ATELLLSN
• Endotoxin: Less than 1 EU/µg of rHuUBE2K, His as determined by LAL method.
• Purity: >95% by SDS-PAGE and HPLC analysis.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 centigrade as supplied. 3 months, -20 to -70 centigrade under sterile conditions after opening.

Gene Information

• Gene Name: UBE2K
• Official Symbol: UBE2K
• Synonyms: UBE2K; ubiquitin-conjugating enzyme E2K; HIP2, huntingtin interacting protein 2 , ubiquitin conjugating enzyme E2K (UBC1 homolog, yeast); ubiquitin-conjugating enzyme E2 K; HYPG; UBC1; HIP-2; E2(25K); ubiquitin-protein ligase; ubiquitin carrier protein; huntingtin interacting protein 2; huntingtin-interacting protein 2; ubiquitin-conjugating enzyme E2-25K; ubiquitin-conjugating enzyme E2(25K); ubiquitin-conjugating enzyme E2-25 KDA; ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast); LIG; HIP2; E2-25K; DKFZp564C1216; DKFZp686J24237
• Gene ID: 3093
• mRNA Refseq: NM_001111112
• Protein Refseq: NP_001104582
• MIM: 602846
• UniProt ID: P61086