Recombinant Human PDGFBB
Cat.No. : | PDGFB-59H |
Product Overview : | Recombinant Human Platelet-Derived Growth Factor BB is a homodimeric, glycosylated, polypeptide chain containing 109 amino acids and having a molecular mass of 32,021 Dalton. PDGF-BB is purified by proprietary chromatographic techniques. |
- Specification
- Gene Information
- Related Products
Cat. No. : | PDGFB-59H |
Description : | PDGF-BB is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. |
Source : | Saccharomyces cerevisiae |
Amino acid sequence : | The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Leu-Gly-Ser-Leu. |
Physical Appearance : | Sterile Filtered White lyophilized (freeze-dried) powder. |
Purity : | Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Formulation : | The protein was lyophilized from 0.2 µm filtered solution in 20mM sodium acetate in absence of any carrier protein. |
Solubility : | It is recommended to reconstitute the lyophilized Platelet-derived Growth Factor-BB in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Biological Activity : | The ED50, calculated by the dose-dependant proliferation of BALB/c 3T3 indicator cells (measured by 3H-thymidine uptake) is < 1 ng/ml, corresponding to a Specific Activity of 1 MIU/mg. |
Protein content : | Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.55 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of PDGF-BB as a Reference Standard. |
Storage : | Lyophilized Platelet-derived Growth Factor BB although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution PDGF BB should be stored at 4℃ between 2-7 days and for future use below -18℃. Please prevent freeze-thaw cycles. |
Gene Name : | PDGFB platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog) [ Homo sapiens ] |
Synonyms : | PDGFB; platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog); SIS; SSV; PDGF2; c-sis; FLJ12858; PDGFB; PDGF subunit B; Platelet-derived growth factor B chain; Platelet-derived growth factor beta; PDGF-2; Platelet-derived growth factor, beta polypeptide (oncogene SIS); platelet-derived growth factor 2 |
Gene ID : | 5155 |
mRNA Refseq : | NM_002608 |
Protein Refseq : | NP_002599 |
UniProt ID : | P01127 |
Chromosome Location : | 22q12.3-q13.1; 22q13.1 |
MIM : | 190040 |
Pathway : | Cytokine-cytokine receptor interaction; Focal adhesion; Gap junction; Glioma; MAPK signaling pathway; Prostate cancer; Regulation of actin cytoskeleton; Hemostasis; Signaling by PDGF |
Function : | cell surface binding; collagen binding; growth factor activity; platelet-derived growth factor binding; platelet-derived growth factor receptor binding; contributes_to platelet-derived growth factor receptor binding; protein heterodimerization activity |
Products Types
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PDGFB-225M | Active Recombinant Mouse PDGFB Protein | +Inquiry |
PDGFB-4410E | Recombinant Equine PDGFB Protein | +Inquiry |
◆ Lysates | ||
PDGFB-1321HCL | Recombinant Human PDGFB cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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