Active Recombinant Human MAP2K4, His-tagged

• Cat.No.: MAP2K4-105H
• Product Overview: Human MKK4, full length, amino acids M1-D399 (as in NCBI/Protein entry NP_003001.1), activated, N-terminal HIS6 fusion protein with a Thrombin cleavage site, expressed in Sf9 insect cells

Specification

• Description: MAP2K4 encodes a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. MAP2K4 is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK1. MAP2K4 is phosphorylated, and thus activated by MAP3K1/MEKK. The knockout studies in mice suggested the roles of this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver.
• Source: Baculovirus infected Sf9
• Species: Human
• Tag: His
• Form: 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 20% glycerol
• Bio-activity: 41 pmol/μg×min
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles!
• Concentration: 0.884 μg/μl

Gene Information

• Gene Name: MAP2K4 mitogen-activated protein kinase kinase 4 [ Homo sapiens ]
• Official Symbol: MAP2K4
• Synonyms: MAP2K4; mitogen-activated protein kinase kinase 4; SERK1; dual specificity mitogen-activated protein kinase kinase 4; JNKK1; MEK4; MKK4; PRKMK4; MEK 4; MAPKK 4; SAPK kinase 1; MAPK/ERK kinase 4; SAPK/ERK kinase 1; MAP kinase kinase 4; JNK-activated kinase 1; JNK activating kinase 1; JNK-activating kinase 1; c-Jun N-terminal kinase kinase 1; stress-activated protein kinase kinase 1; JNKK; SEK1; MAPKK4; SAPKK1; SAPKK-1
• Gene ID: 6416
• mRNA Refseq: NM_003010
• Protein Refseq: NP_003001
• MIM: 601335
• UniProt ID: P45985
• Chromosome Location: 17p12
• Pathway: Activated TLR4 signalling, organism-specific biosystem; Apoptosis, organism-specific biosystem; CD40/CD40L signaling, organism-specific biosystem; CDC42 signaling events, organism-specific biosystem; Cellular roles of Anthrax toxin, organism-specific biosystem; Ceramide signaling pathway, organism-specific biosystem; Chagas disease (American trypanosomiasis), organism-specific biosystem
• Function: ATP binding; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; protein tyrosine kinase activity