Recombinant Human GAPDH, GST-tagged

• Cat.No.: GAPDH-117H
• Product Overview: Human GAPDH full-length ORF (1 a.a. - 335 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. Two transcript variants encoding different isoforms have been found for this gene.
• Source: Wheat germ
• Species: Human
• Tag: GST
• Molecular Mass: 62.59 kDa
• AA Sequence: MGKVKVGVNGFGRIGRLVTRAAFNS GKVDIVAINDPFIDLNYMVYMFQYD STHGKFHGTVKAENGKLVINGNPIT IFQERDPSKIKWGDAGAEYVVESTG VFTTMEKAGAHLQGGAKRVIISAPS ADAPMFVMGVNHEKYDNSLKIISN ASCTTNCLAPLAKVIHDNFGIVEGL MTTVHAITATQKTVDGPSGKLWRDG R GALQNIIPASTGAAKAVGKVIPE LNGKLTGMAFRVPTANVSVVDLTCR LEKPAKYDDIKKVVKQASEGPLKGI LGYTEHQVVSSDFNSDTHSSTFDAG AG IALNDHFVKLISWYDNEFGYSNRVV DLMAHMASKE
• Applications: ELISA; WB-Re; AP; Array
• Storage: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: GAPDH glyceraldehyde-3-phosphate dehydrogenase [ Homo sapiens (human) ]
• Official Symbol: GAPDH
• Synonyms: GAPDH; G3PD; GAPD; glyceraldehyde-3-phosphate dehydrogenase; aging-associated gene 9 protein; peptidyl-cysteine S-nitrosylase GAPDH; EC 1.2.1.12
• Gene ID: 2597
• mRNA Refseq: NM_002046
• Protein Refseq: NP_002037
• MIM: 138400
• UniProt ID: P04406
• Chromosome Location: 12p13
• Pathway: Alzheimer''s disease; Biosynthesis of amino acids; Carbon metabolism
• Function: NAD binding; glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity; identical protein binding