Recombinant Human CD200 /OX-2 Protein, With C-Fc Tag (rhCD200 Fc Chimera) Gln 31 - Gly 232 was produced in human 293 cells (HEK293).
CD200 also known as OX-2 membrane glycoprotein (OX-2), is a type-1 membrane glycoprotein, which contains two immunoglobulin domains (1 Ig-like C2-type (immunoglobulin-like) domain and Ig-like V-type (immunoglobulin-like) domain), and thus belongs to the immunoglobulin superfamily. CD200 / OX-2 is widely expressed in multiple cell types. CD200 interacts with a structurally related receptor (CD200R) expressed mainly on myeloid cells and is involved in regulation of macrophage and mast cell function. OX-2 / CD200 and CD200R associate via their respective N-terminal Ig-like domains. CD200 also plays an important role in prevention of graft rejection, autoimmune diseases and spontaneous abortion.
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.0. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Measured by its binding ability in a functional ELISA. Immobilized recombinant human CD200R1 at 2 μg/ml,the concentration of rhCD200 Fc Chimera that produces 50% of the optimal binding response is appoximately 2.0?16 ng/ml.
rhCD200 Fc Chimera, fused with Fc fragment of human IgG1 at the C-terminus, has a calculated MW of 48.6 kDa. The predicted N-terminus is Gln 31. DTT-reduced Protein migrates as 50-68 kDa due to glycosylation.
Less than 1.0 EU per μg of the rhCD200 Fc Chimera by the LAL method.
>95% as determined by SDS-PAGE.
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4oC-8oC); After reconstitution under sterile conditions for 1 month (4oC-8oC) or 3 months (-20oC to -70oC).