Recombinant Human HLA-F, His-tagged

• Cat.No.: HLA-F-239H
• Product Overview: HLA-F, 22-305aa, Human, His tag, E.coli

Specification

• Description: HLA class I histocompatibility antigen, alpha chain F isoform 1 precursor, also known as HLA-F, is member of the MHC family that are specifically involved in the presentation of antigens to the T cell receptor. There are two classes of HLA antigens. HLA-F, belong to the Class I molecules. Class I molecules are expressed in nearly all cells and play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum. Recombinant human HLA-F, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
• Molecular Mass: 35.1 kDa (307aa) confirmed by MALDI-TOF
• Protein length: 22-305aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSGSHSLRY FSTAVSRPGR GEPRYIAVEY VDDTQFLRFD SDAAIPRMEP REPWVEQEGP QYWEWTTGYA KANAQTDRVA LRNLLRRYNQ SEAGSHTLQG MNGCDMGPDG RLLRGYHQHA YDGKDYISLN EDLRSWTAAD TVAQITQRFY EAEEYAEEFR TYLEGECLEL LRRYLENGKE TLQRADPPKA HVAHHPISDH EATLRCWALG FYPAEITLTW QRDGEEQTQD TELVETRPAG DGTFQKWAAV VVPPGEEQRY TCHVQHEGLP QPLILRWEQS PQPTIPI
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: HLA-F major histocompatibility complex, class I, F [ Homo sapiens ]
• Official Symbol: HLA-F
• Synonyms: HLA-F; major histocompatibility complex, class I, F; HLA class I histocompatibility antigen, alpha chain F; HLA F antigen; leukocyte antigen F; HLA class I molecule; MHC class Ib antigen; MHC class I antigen F; HLAF; CDA12; HLA-5.4; HLA-CDA12
• Gene ID: 3134
• mRNA Refseq: NM_001098478
• Protein Refseq: NP_001091948
• MIM: 143110
• UniProt ID: P30511
• Chromosome Location: 6p21.3
• Pathway: Adaptive Immune System, organism-specific biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Antigen Presentation: Folding, assembly and peptide loading of class I MHC, organism-specific biosystem; Antigen processing and presentation, organism-specific biosystem; Antigen processing and presentation, conserved biosystem; Antigen processing-Cross presentation, organism-specific biosystem
• Function: MHC class I receptor activity