Recombinant Human POLR1C, His-tagged

• Cat.No.: POLR1C-260H
• Product Overview: POLR1C, 1-349aa, Human, His tag, E.coli

Specification

• Description: POLR1C is a subunit of both RNA polymerase I and RNA polymerase III complexes. The encoded protein is part of the Pol core element. Defects in this gene have been associated with Treacher Collins syndrome (TCS). Recombinant human POLR1C protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
• Molecular Mass: 41.6 kDa (369aa) confirmed by MALDI-TOF
• Protein length: 1-349aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAASQAV EEMRSRVVLG EFGVRNVHTT DFPGNYSGYD DAWDQDRFEK NFRVDVVHMD ENSLEFDMVG IDAAIANAFR RILLAEVPTM AVEKVLVYNN TSIVQDEILA HRLGLIPIHA DPRLFEYRNQ GDEEGTEIDT LQFRLQVRCT RNPHAAKDSS DPNELYVNHK VYTRHMTWIP LGNQADLFPE GTIRPVHDDI LIAQLRPGQE IDLLMHCVKG IGKDHAKFSP VATASYRLLP DITLLEPVEG EAAEELSRCF SPGVIEVQEV QGKKVARVAN PRLDTFSREI FRNEKLKKVV RLARVRDHYI FSVESTGVLP PDVLVSEAIK VLMGKCRRFL DELDAVQMD
• Purity: >80% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: POLR1C polymerase (RNA) I polypeptide C, 30kDa [ Homo sapiens ]
• Official Symbol: POLR1C
• Synonyms: POLR1C; polymerase (RNA) I polypeptide C, 30kDa; DNA-directed RNA polymerases I and III subunit RPAC1; RPA5; RPA39; RPA40; RPAC1; AC40; RPC40; RNA polymerases I and III subunit AC1; DNA-directed RNA polymerase I subunit C; DNA-directed RNA polymerases I and III 40 kDa polypeptide; TCS3; RP3-337H4.4
• Gene ID: 9533
• mRNA Refseq: NM_203290
• Protein Refseq: NP_976035
• MIM: 610060
• UniProt ID: O15160
• Chromosome Location: 6p21.1
• Pathway: Cytosolic DNA-sensing pathway, organism-specific biosystem; Cytosolic DNA-sensing pathway, conserved biosystem; Gene Expression, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Purine metabolism, organism-specific biosystem; Purine metabolism, conserved biosystem; Pyrimidine metabolism, organism-specific biosystem
• Function: DNA binding; DNA-directed RNA polymerase activity; protein dimerization activity