Human Platelet Factor 4

• Cat.No.: PF4-52H
• Product Overview: Human PF-4 a7.8 kDa protein consisting of 70 amino acid residues.

Specification

• Cat. No.: PF4-52H
• Description: Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair. Oncostatin-A is a member of the CXC chemokine family. Human PF4 is used for the proof of heparin-induced thrombocytopenia. Furthermore it is used as an inhibitor in the angiogenesis during tumor therapy.
• Source: Human Platelets.
• Amino Acid Sequence: The sequence of the first four N-terminal amino acids was determined and was found to be Glu-Ala-Glu-Glu.
• Physical Appearance: Sterile Filtered white lyophilized powder.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: The protein was lyophilized in PBS buffer.
• Solubility: It is recommended to reconstitute the lyophilized CXCL4 in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
• Activity Control: Binding of HIT-antibodies.
• Stability: Human CXCL4 although stable at 25℃ 1 week, should be stored desiccated below -18℃. Please prevent freeze-thaw cycles.
• Publication:
  Interactions of Spike-RBD of SARS-CoV-2 and Platelet Factor 4: New Insights in the Etiopathogenesis of Thrombosis (2021)

Gene Information

• Gene Name: PF4 platelet factor 4 [ Homo sapiens ]
• Synonyms: PF4; platelet factor 4; CXCL4; SCYB4; MGC138298; Iroplact; Oncostatin-A; C-X-C motif chemokine 4; chemokine (C-X-C motif) ligand 4
• Gene ID: 5196
• mRNA Refseq: NM_002619
• Protein Refseq: NP_002610
• MIM: 173460
• UniProt ID: P02776
• Chromosome Location: 4q12-q21
• Pathway: Chemokine signaling pathway; Cytokine-cytokine receptor interaction; Hemostasis; Signaling by GPCR
• Function: chemokine activity; heparin binding