Active Recombinant Human ICAM1

• Cat.No.: ICAM1-89H
• Product Overview: Recombinant Human ICAM1 (Met1-Glu480) was expressed in Mouse NSO Cells.

Specification

• Description: This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor.
• Source: Mammalian cells
• Species: Human
• Predicted N Terminal: Predicted Gln28
• Form: Lyophilized from a 0.2 μm filtered solution in PBS, containing Calcium and Magnesium with Sorbitol and Trehalose.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5 x 104 cells/well are added to Recombinant Human ICAM 1/CD54 coated plates (12.5 μg/mL with 100 μL/well), >30% cells will adhere after 1 hour incubation at 37 °C. Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Predicted Molecular Mass: 50 kDa SDS-PAGE: 70-90 kDa, reducing conditions
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 400 μg/mL in sterile PBS.

Gene Information

• Gene Name: ICAM1 intercellular adhesion molecule 1 [ Homo sapiens (human) ]
• Official Symbol: ICAM1
• Synonyms: ICAM1; BB2; CD54; P3.58; intercellular adhesion molecule 1; intercellular adhesion molecule 1; ICAM-1; cell surface glycoprotein P3.58; major group rhinovirus receptor; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
• Gene ID: 3383
• mRNA Refseq: NM_000201
• Protein Refseq: NP_000192
• MIM: 147840
• UniProt ID: P05362
• Chromosome Location: 19p13.3-p13.2
• Pathway: Adaptive Immune System; African trypanosomiasis; Cell adhesion molecules (CAMs)
• Function: integrin binding; receptor activity; transmembrane signaling receptor activity