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Home > Products > Recombinant Proteins > Recombinant Mouse Cd40, His-tagged

Recombinant Mouse Cd40, His-tagged

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Cat.No.:
Cd40-86M
Product Overview:
Optimized DNA sequence encoding extracellular domain of Mouse CD40 including a C-terminal His tag was expressed in HEK293 cells.
Description:
The CD40 molecule is a 45- 50-kDa type I phosphorylated glycoprotein that belongs to the tumor necrosis factor receptor superfamily and was initially considered to be a growth factor receptor on B cells. The interaction of CD40 with its natural ligand, CD154, is a crucial step in T cell-dependent B cell activation and differentiation. The cellular events triggered in CD40-positive cells following CD40/CD154 interactions are not restricted to B cells only but can also be triggered in dendritic cells, monocytes, epithelial cells, endothelial cells, and fibroblasts. Thus, CD40 ligation mediates a broad variety of immune and inflammatory responses.
Source:
HEK293 cells
Species:
Mouse
Tag:
His
Form:
Recombinant Mouse CD40 is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Bio-activity:
The activity was tested by the ability of immobilized recombinant mouse CD40 to bind Human CD40 Ligand with a linear range of 10-500 ng/ml.
Molecular Mass:
Recombinant CD40 is a protein consisting of 180 amino acid residue subunits, due to glycosylation migrates as an approximately 30kDa protein on SDS-PAGE.
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Purity:
>97%, as determined by SDS-PAGE and HPLC
Storage:
Recombinant CD40 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months. Avoid repeated freeze/thaw cycles.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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