Active Recombinant Mouse Cd40, His-tagged

• Cat.No.: Cd40-86M
• Product Overview: Optimized DNA sequence encoding extracellular domain of Mouse CD40 including a C-terminal His tag was expressed in HEK293 cells.

Specification

• Description: The CD40 molecule is a 45- 50-kDa type I phosphorylated glycoprotein that belongs to the tumor necrosis factor receptor superfamily and was initially considered to be a growth factor receptor on B cells. The interaction of CD40 with its natural ligand, CD154, is a crucial step in T cell-dependent B cell activation and differentiation. The cellular events triggered in CD40-positive cells following CD40/CD154 interactions are not restricted to B cells only but can also be triggered in dendritic cells, monocytes, epithelial cells, endothelial cells, and fibroblasts. Thus, CD40 ligation mediates a broad variety of immune and inflammatory responses.
• Source: HEK293 cells
• Species: Mouse
• Tag: His
• Form: Recombinant Mouse CD40 is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
• Bio-activity: The activity was tested by the ability of immobilized recombinant mouse CD40 to bind Human CD40 Ligand with a linear range of 10-500 ng/ml.
• Molecular Mass: Recombinant CD40 is a protein consisting of 180 amino acid residue subunits, due to glycosylation migrates as an approximately 30kDa protein on SDS-PAGE.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
• Purity: >97%, as determined by SDS-PAGE and HPLC
• Storage: Recombinant CD40 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months. Avoid repeated freeze/thaw cycles.

Gene Information

• Gene Name: Cd40 CD40 antigen [ Mus musculus (house mouse) ]
• Official Symbol: Cd40
• Synonyms: Cd40; IGM; p50; Bp50; GP39; IMD3; TRAP; HIGM1; T-BAM; Tnfrsf5; AI326936; CD40 antigen; tumor necrosis factor receptor superfamily member 5; CD40L receptor; B-cell surface antigen CD40; T-cell differentiation antigen; tumor necrosis factor receptor superfamily, member 5
• Gene ID: 21939
• mRNA Refseq: NM_011611
• Protein Refseq: NP_035741
• UniProt ID: P27512
• Chromosome Location: 2 H3; 2 85.38 cM
• Pathway: Allograft rejection; Autoimmune thyroid disease; Epstein-Barr virus infection
• Function: antigen binding; enzyme binding; protein binding