Recombinant Human MDM2, GST-tagged

• Cat.No.: MDM2-1065H
• Product Overview: Recombinant Human MDM2 (1 a.a. - 491 a.a.), fused with GST-tag at N-terminal, was expressed in wheat germ.

Specification

• Description: This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.
• Source: Wheat germ
• Species: Human
• Tag: GST
• Molecular Mass: 81.6 kDa
• AA Sequence: MCNTNMSVPTDGAVTTSQIPASEQE TLVRPKPLLLKLLKSVGAQKDTYTM KEVLFYLGQYIMTKRLYDEKQQHIV YCSNDLLGDLFGVPSFSVKEHRKIY TMIYRNLVVVNQQESSDSGTSVSEN RCHLEGGSDQKDLVQELQEEKPSSS HLVSRPSTSSRRRAISETEENSDEL SGERQRKRHKSDSISLSFDESLALC VIREICCERSSSSESTGTPSNPDLD AGVSEHSGDWLDQDSVSDQFSVEFE VESLDSEDYSLSEEGQELSDEDDEV YQVTVYQAGESDTDSFEEDPEISLA DYWKCTSCNEMNPPLPSHCNRCWAL RENWLPEDKGKDKGEISEKAKLENS TQAEEGFDVPDCKKTIVNDSRESCV EENDDKITQASQSQESEDYSQPSTS SSIIYSSQEDVKEFEREETQDKEES VESSLPLNAIEPCVICQGRPKNGCI VHGKTGHLMACFTCAKKLKKRNKPC PVCRQPIQMIVLTYFP
• Applications: ELISA; WB-Re; AP; Array
• Storage: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: MDM2 MDM2 oncogene, E3 ubiquitin protein ligase [ Homo sapiens (human) ]
• Official Symbol: MDM2
• Synonyms: MDM2; HDMX; hdm2; ACTFS; MDM2 oncogene, E3 ubiquitin protein ligase; E3 ubiquitin-protein ligase Mdm2; oncoprotein Mdm2; Mdm2, p53 E3 ubiquitin protein ligase homolog; double minute 2, human homolog of; p53-binding protein; Mdm2, transformed 3T3 cell double minute 2, p53 binding protein
• Gene ID: 4193
• mRNA Refseq: NM_001145337
• Protein Refseq: NP_001138809
• MIM: 164785
• UniProt ID: Q00987
• Chromosome Location: 12q14.3-q15
• Pathway: AKT phosphorylates targets in the cytosol; Aurora A signaling; Cell Cycle Checkpoints
• Function: enzyme binding; identical protein binding; peroxisome proliferator activated receptor binding