| Description : |
The bone morphogenetic proteins are a family of secreted signalling molecules that can induce ectopic bone growth. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Bone morphogenetic protein 7 (BMP-7), also known as osteogenic protein 1 (OP1), is a widely expressed TGFb superfamily member with important functions during embryogenesis, in the adult, and in disease (Chen et al., 2004, Kishigami and Mishina 2005). BMP-7 plays a role in a variety of organ systems. It promotes new bone formation and nephron development (Sampath et al., 1992, Kazama et al., 2008), inhibits the branching of prostate epithelium (Grishina et al., 2005), and antagonizes epithelialmesenchymal transition (Zeisberg et al., 2003, Yu et al., 2009). In pathological conditions, BMP7 inhibits tumor growth and metastasis (Buijs et al., 2007), ameliorates fibrotic damage in nephritis (Zeisberg et al., 2003), and promotes neuroregeneration following brain ischemia (Chou et al., 2006). |
| Source : |
Nicotiana benthamiana |
| Species : |
Human |
| Form : |
Lyophilized from a Tris HCl 0.05M buffer at pH 7.4. |
| Bio-activity : |
The biological activity of BMP-7 is measured by its ability to induce alkaline phosphatase production by ATDC5 cells. ED50 ≤ 40ng/ml |
| Molecular Mass : |
Recombinant human BMP-7 is a protein composed of 16.5 kDa single chain, containing 144 amino residues. |
| AA Sequence : |
HHHHHHSTGSKQRSQNRSKTPKNQE ALRMANVAENSSSDQRQACKKHELY VSFRDLGWQDWIIAPEGYAAYYCEG ECAFPLNSYMNATNHAIVQTLVHFI NPETVPKPCCAPTQLNAISVLYFDD SSNVILKKYRNMVVRACGCH |
| Endotoxin : |
< 0.04 eu ug protein (lal> |
| Purity : |
>97% by SDS-PAGE gel |
| Applications : |
Cell culture, Western blot |
| Storage : |
This lyophilized preparation is stable at 2-8o C for short term, long storage it should be kept at -20oC. Reconstituted protein should be stored in working aliquots at –20°C and it is recommended to add a carrier protein (0.1% HSA or BSA). Repeated freezing and thawing is not recommended. |
| Reconstitution : |
Lyophilized protein should be reconstituted in water to a concentration of 50 ng/ul. Upon reconstitution, It can be stored in working aliquots at –20°C for future use. Optimal reconstitution please follow batch Quality Control sheet instructions. |
