Active Recombinant Human KIT Mutant (Y823D), GST-tagged

• Cat.No.: KIT-88H
• Product Overview: Recombinant human c-KIT (Y823D) (544-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: c-KIT (Y823D) encodes the ligand-binding chain (alpha) of the gamma interferon receptor which is a heterodimer of IFNGR1 and IFNGR2. c-KIT (Y823D) is a regulator of hematopoietic stem cells during homeostasis and under conditions of infectious stress. A genetic variation in c-KIT (Y823D) is associated with susceptibility to Helicobacter pylori infection. In addition, defects in c-KIT (Y823D) are a cause of mendelian susceptibility to mycobacterial disease, also known as familial disseminated atypical mycobacterial infection. c-KIT (Y823D is also associated with the clinical outcome of HBV infection in Chinese adults.
• Source: Sf9 insect cells using baculovirus
• Species: Human
• Tag: GST
• Form: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: The specific activity was determined to be 5 nmol/min/mg
• Molecular Mass: ~73 kDa
• Purity: >70% by densitometry.
• Applications: Kinase Assay
• Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.05 μg/μl

Gene Information

• Gene Name: KIT v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog [ Homo sapiens ]
• Official Symbol: KIT
• Synonyms: KIT; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; PBT, piebald trait; mast/stem cell growth factor receptor Kit; C Kit; CD117; SCFR; p145 c-kit; proto-oncogene c-Kit; piebald trait protein; soluble KIT variant 1; tyrosine-protein kinase Kit; proto-oncogene tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein; PBT; C-Kit
• Gene ID: 3815
• mRNA Refseq: NM_000222
• Protein Refseq: NP_000213
• MIM: 164920
• UniProt ID: P10721
• Chromosome Location: 4q11-q12
• Pathway: Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; C-MYB transcription factor network, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Endocytosis, organism-specific biosystem; Endocytosis, conserved biosystem
• Function: ATP binding; cytokine binding; metal ion binding; nucleotide binding; protease binding; protein binding; protein homodimerization activity; protein tyrosine kinase activity; receptor activity; receptor signaling protein tyrosine kinase activity; stem cell factor receptor activity; transmembrane receptor protein tyrosine kinase activity