Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-097-1828   Email: info@creative-biomart.com
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Native Human β-thrombin

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Bovine(4)
Human(5)
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Biotin(1)
N/A(3)
Cat.No.:
Thrombin-25H
Product Overview:
Human beta-thrombin are prepared from purified a-thrombin by limited proteolysis with TPCK-treated trypsin, essentially by the method of Braun et al.
Description:
Alpha-thrombin is a highly specific serine protease generated by proteolytic activation of the zymogen prothrombin. Purified forms of α-thrombin have been shown to undergo autolysis upon long term storage to less active forms. Similar inactivation was observed upon limited digestion of a-thrombin with trypsin. These proteolyzed forms of α-thrombin have been termed β-thrombin and γ-thrombin. Human β-thrombin is generated by cleavage of the B-chain at the Arg106-Tyr107 bond. γ-thrombin is generated by further cleavage of the β-thrombin B2-chain at the Lys190-Gly191 bond. Another form of proteolyzed thrombin, termed β-thrombin is formed by the single cleavage of thrombin at Arg-154. γ-thrombin is produced by proteolytic cleavage at both of these sites (Arg70/73 and Arg-154) in the B-chain. These cleavages cause release of peptides that are no longer covalently attached to the thrombin molecule, but remain associated through ion exchange and gel filtration chromatography. These proteolyzed forms of thrombin retain their ability to cleave small synthetic substrates and some protein substrates such as factor XIII, antithrombin III and prothrombin. Their ability to clot fibrinogen, cleave thrombospondin or activate protein C have been markedly decreased.
Source:
Plasma
Species:
Human
Form:
10 mM NaPO4, 0.3 M NaCl, pH 6.5
Bio-activity:
<5% thrombin>
Purity:
≥95% by SDS-PAGE. NOT tissue/cell culture grade.
Storage:
at or below -70 °C
Concentration:
6.4 mg/mL


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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