Recombinant human Activin AB, Active, His-tagged

• Cat.No.: INHBA&INHBB-1544H
• Product Overview: Recombinant Activin AB is a disulphide linked heterodimer of subunits βA / βB . βA Single chain, containing 116 aa (13.7 kDa) and βB single chain, 123 amino residues (14kDa). Recombinant human Activin AB contains a His-tag at the N-terminal end.

Specification

• Description: Activins are homodimers or heterodimers of the various β subunit isoforms, belonging to the TGFβ family. Mature Activin AB has two chains of 116 and 123 amino acids residues (βA-βB). Activin exhibits a wide range of biological activities, including mesoderm induction, neural cell differentiation, bone remodelling, haematopoiesis, and reproductive physiology. Activins plays a key role in the production and regulation of hormones such as FSH, LH, GnRH and ACTH. Inhibins /Activins are proteins that are formed by the dimerization of two subunits, i. e. an α with either βA –inhibin A- or βB - inhibin B. The subunits βA and βB can also form homodimers or heterodimers calleds activins: Activin A (βAβA), Activin B (βBβB) and Activin AB (βAβB). The activin gene family comprises the additional, but poorly characterized members activin βC, βD, and βE.As with other members of the super-family, Activins interact with two types of cell surface trans-membrane receptors (Types I and II) which have intrinsic serine/threonine kinase activities in their cytoplasmic domains, Activin type 1 receptors, ACVR1, ACVR1B, ACVR1C and Activin type 2 receptors, ACVR2A, ACVR2B.The development of assays distinguishing between different forms of activins and inhibins, along with knock-in and knock-out models, have provided evidence that the betaA- and betaB-subunits have independent and separate roles physiologically. Additionally, evaluation of ligand-receptor interactions indicates significant differences in receptor affinity between activin isoforms, as well as between inhibin isoforms.
• Source: Human recombinant protein expressed in Nicotiana benthamiana. It is produced by transient expression in non-transgenic plants. This product contains no animal–derived components or impurities. Animal Free product.
• Species: Human
• Tag: His
• Form: Recombinant human Activin is lyophilized from a Tris HCl 0.05M buffer at pH 7.4.
• Molecular Mass: 13.7 kDa, 14kDa
• AA Sequence: βA:GLECDGKVNICCKKQFFVSFKDIGW NDWIIAPSGYHANYCEGECPSHIAG TSGSSLSFHSTVINHYRMRGHSPFA NLKSCCVPTKLRPMSMLYYDDGQNI IKKDIQNMIVEECGCSβB:GLECDGRTNLCCRQQFFIDFRLIGW NDWIIAPTGYYGNYCEGSCPAYLAG VPGSASSFHTAVVNQYRMRGLNPGT VNSCCIPTKLSTMSMLYFDDEYNIV KRDVPNMIVEECGCA
• Endotoxin: < 0.04 EU/µg protein (LAL method)
• Purity: >97% by SDS-PAGE gel
• Storage: This lyophilized preparation is stable at 2-8º C for short term, long storage it should be kept at -20ºC. Reconstituted protein should be stored in working aliquots at –20°C. Repeated freezing and thawing is not recommended.
• Reconstitution: Centrifuge vial before opening. Lyophilized protein should be reconstituted in water to a concentration of 50 ng/μl. Optimal concentration should be determined for specific application and cell lines.

Gene Information

• Gene Name: INHBA inhibin, beta A [ Homo sapiens ]
• Official Symbol: INHBA
• Synonyms: INHBA; inhibin, beta A; inhibin, beta A (activin A, activin AB alpha polypeptide); inhibin beta A chain; Inhibin, beta-1; activin beta-A chain; FSH-releasing protein; erythroid differentiation factor; erythroid differentiation protein; follicle-stimulating hormone-releasing protein; EDF; FRP
• Gene ID: 3624
• mRNA Refseq: NM_002192
• Protein Refseq: NP_002183
• MIM: 147290
• UniProt ID: P08476
• Chromosome Location: 7p15-p13
• Pathway: ALK1 signaling events, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Glycoprotein hormones, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of amino acids and derivatives, organism-specific biosystem; Peptide hormone biosynthesis, organism-specific biosystem
• Function: cytokine activity; follistatin binding; growth factor activity; hormone activity; identical protein binding; protein binding; protein heterodimerization activity; type II activin receptor binding