Active Recombinant Human KHK, His-tagged

• Cat.No.: KHK-450H
• Product Overview: Recombinant Human KHK (Met1-Val298), fused with C-terminal 6-His tag, was expressed in E. coli.

Specification

• Description: This gene encodes ketohexokinase that catalyzes conversion of fructose to fructose-1-phosphate. The product of this gene is the first enzyme with a specialized pathway that catabolizes dietary fructose. Alternatively spliced transcript variants encoding different isoforms have been identified.
• Source: E. coli
• Species: Human
• Tag: His
• Predicted N Terminal: Met1
• Form: Supplied as a 0.2 µm filtered solution in Tris, NaCl, Brij-35 and DTT.
• Bio-activity: Measured by its ability to phosphorylate D-(-)-fructose. The specific activity is >1,100 pmol/min/µg.
• Molecular Mass: Predicted Molecular Mass: 34 kDa SDS-PAGE: 28-32 kDa, reducing conditions
• Endotoxin: < 1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
• Storage: Avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

Gene Information

• Gene Name: KHK ketohexokinase (fructokinase) [ Homo sapiens (human) ]
• Official Symbol: KHK
• Synonyms: KHK; ketohexokinase (fructokinase); ketohexokinase; hepatic fructokinase; NP_000212.1; EC 2.7.1.3; NP_006479.1
• Gene ID: 3795
• mRNA Refseq: NM_000221
• Protein Refseq: NP_000212
• MIM: 614058
• UniProt ID: P50053
• Chromosome Location: 2p23.3
• Pathway: Fructose and mannose metabolism; Fructose catabolism; sucrose degradation V (mammalian)
• Function: ATP binding; ketohexokinase activity