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Recombinant Human MIA protein

Cat.No. : MIA-140H
Product Overview : Recombinant Human MIA protein was expressed in Escherichia coli.
  • Specification
  • Gene Information
  • Related Products
Source : E.coli
Species : Human
Form : Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4, with 5 % Trehalose.
Bio-activity : Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human A375 cell line is less than 5 μg/ml, corresponding to a specific activity of > 200 IU/mg.
Molecular Mass : Approximately 12.1 kDa, a single non-glycosylated polypeptide chain containing 107 amino acids.
Protein length : 107
AA Sequence : GPMPKLADRKLCADQECSHPISMAV ALQDYMAPDCRFLTIHRGQVVYVFS KLKGRGRLFWGGSVQGDYYGDLAAR LGYFPSSIVREDQTLKPGKVDVKTD KWDFYCQ
Endotoxin : Less than 0.1 EU/µg of rHuMIA as determined by LAL method.
Purity : >98% by SDS-PAGE and HPLC analysis.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.
Gene Name : MIA
Official Symbol : MIA
Synonyms : MIA; melanoma inhibitory activity; melanoma-derived growth regulatory protein; CD RAP; CD-RAP;
Gene ID : 8190
mRNA Refseq : NM_001202553
Protein Refseq : NP_001189482
MIM : 601340
UniProt ID : Q16674

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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