Recombinant Human ERBB2, StrepII-tagged

• Cat.No.: ERBB2-229H
• Product Overview: Purified human recombinant Receptor tyrosine-protein kinase erbB-2 HER2 (ERBB2) protein (amino acids 23-652, 630 a.a.) with StrepII tag, produced in human cells. Predicted molecular weight: 69.3 kDa. (Accession NP_004439.2; UniProt P04626)

Specification

• Description: ERBB2 is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase.
• Source: Human Cells
• Species: Human
• Tag: StrepII
• Form: Lyophilized from sterile 0.2μm filtered solution in 0.3X PBS with 10% Trehalose (carrier-free).
• Protein length: 23-652, 630 a.a.
• AA Sequence: TQVCTGTDMKLRLPASPETHLDMLR HLYQGCQVVQGNLELTYLPTNASLS FLQDIQEVQGYVLIAHNQVRQVPLQ RLRIVRGTQLFEDNYALAVLDNGDP LNNTTPVTGASPGGLRELQLRSLTE ILKGGVLIQRNPQLCYQDTILWKDI FHKNNQLALTLIDTNRSRACHPCSP MCKGSRCWGESSEDCQSLTRTVCAG GCARCKGPLPTDCCHEQCAAGCTGP KHSDCLACLHFNHSGICELHCPALV TYNTDTFESMPNPEGRYTFGASCVT ACPYNYLSTDVGSCTLVCPLHNQEV TAEDGTQRCEKCSKPCARVCYGLGM EHLREVRAVTSANIQEFAGCKKIFG SLAFLPESFDGDPASNTAPLQPEQL QVFETLEEITGYLYISAWPDSLPDL SVFQNLQVIRGRILHNGAYSLTLQG LGISWLGLRSLRELGSGLALIHHNT HLCFVHTVPWDQLFRNPHQALLHTA NRPEDECVGEGLACHQLCARGHCWG PGPTQCVNCSQFLRGQECVEECRVL QGLPREYVNARHCLPCHPECQPQNG SVTCFGPEADQCVACAHYKDPPFCV ARCPSGVKPDLSYMPIWKFPDEEGA CQPCPINCTHSCVDLDDKGCPAEQR ASPLT
• Endotoxin: <0.1 eu per ug protein by lal
• Purity: >95% pure by SDS-PAGE
• Storage: 12 months at -20°C as supplied; 1 month at 4°C after reconstitution. Avoid freeze/thaw cycles.
• Reconstitution: Centrifuge the vial prior to opening. Reconstitute in PBS to a concentration of 0.1 mg/ml.

Gene Information

• Gene Name: ERBB2 v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) [ Homo sapiens ]
• Official Symbol: ERBB2
• Synonyms: ERBB2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian); NGL, v erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog); receptor tyrosine-protein kinase erbB-2; CD340; HER 2; HER2; NEU; herstatin; p185erbB2; proto-oncogene Neu; c-erb B2/neu protein; proto-oncogene c-ErbB-2; metastatic lymph node gene 19 protein; tyrosine kinase-type cell surface receptor HER2; neuroblastoma/glioblastoma derived oncogene homolog; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog); NGL; TKR1; HER-2; MLN 19; HER-2/neu
• Gene ID: 2064
• mRNA Refseq: NM_001005862
• Protein Refseq: NP_001005862
• MIM: 164870
• UniProt ID: P04626
• Chromosome Location: 17q11.2-q12
• Pathway: Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Axon guidance, organism-specific biosystem; Bladder cancer, organism-specific biosystem; Bladder cancer, conserved biosystem; Calcium signaling pathway, organism-specific biosystem
• Function: ATP binding; ErbB-3 class receptor binding; Hsp90 protein binding; RNA polymerase I core binding; epidermal growth factor-activated receptor activity; glycoprotein binding; contributes_to growth factor binding; identical protein binding; nucleotide binding; protein C-terminus binding; protein binding; protein dimerization activity; protein heterodimerization activity; protein heterodimerization activity; protein heterodimerization activity; protein phosphatase binding; protein tyrosine kinase activity; protein tyrosine kinase activity; protein tyrosine kinase activity; receptor activity; receptor signaling protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; transmembrane signaling receptor activity; ubiquitin protein ligase binding