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Active Native Streptomyces griseus Protease

Cat.No. : Protease-20S
Product Overview : It is collected from culture broth of S. griseus and purified by successive column procedures.
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Description : A protease (also termed peptidase or proteinase) is any enzyme that performs proteolysis, that is, begins protein catabolism by hydrolysis of the peptide bonds that link amino acids together in the polypeptide chain forming the protein. Proteases have evolved multiple times, and different classes of protease can perform the same reaction by completely different catalytic mechanisms. Proteases can be found in animals, plants, bacteria, archea and viruses.
Source : Streptomyces griseus
Species : S. griseus
Form : White to tan powder
Bio-activity : Approximately 4 units per mg solid.
Unit Definition : One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
Characteristic : Highly stable in pH range of 5.0 to 9.0, with peak activity at pH 8.8; Compatible with many DNA and RNA isolation buffers; Broad substrate specificity.
Applications : The enzyme has been used for the digestion and analysis of antithrombin-heparin complexes. It has also been used for the isolation of enzyme-resistant starch. This enzyme is more active at a higher pH range than the known alkaline protease, showing the proteolytic activity even in 0.2N NaOH solution. This enzyme is useful for proteolysis of insoluble protein and for structure investigation of protein.
Stability : Completely inactivated by heating above 80 °C for 15-20 minutes.

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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