Recombinant Human protein kinase, AMP-activated, beta 1 non-catalytic subunit, His-tagged
Cat.No. : | PRKAB1-347H |
Product Overview : | PRKAB1, 1-270aa, Human, His tag, E.coli |
- Specification
- Gene Information
- Related Products
Description : | 5"-AMP-activated protein kinase subunit beta-1, also known as PRKAB1, inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3). Recombinant human PRKAB1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
Source : | E.coli |
Species : | Human |
Tag : | His-tag |
Form : | Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT |
Molecular Mass : | 32.8 kDa (293aa) confirmed by MALDI-TOF(Molecular size on SDS-PAGE will appear higher) |
AA Sequence : | MGSSHHHHHH SSGLVPRGSH MGSMGNTSSE RAALERHGGH KTPRRDSSGG TKDGDRPKIL MDSPEDADLF HSEEIKAPEK EEFLAWQHDL EVNDKAPAQA RPTVFRWTGG GKEVYLSGSF NNWSKLPLTR SHNNFVAILD LPEGEHQYKF FVDGQWTHDP SEPIVTSQLG TVNNIIQVKK TDFEVFDALM VDSQKCSDVS ELSSSPPGPY HQEPYVCKPE ERFRAPPILP PHLLQVILNK DTGISCDPAL LPEPNHVMLN HLYALSIKDG VMVLSATHRY KKKYVTTLLY KPI |
Purity : | >85% by SDS - PAGE |
Storage : | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
Concentration : | 0.5 mg/ml (determined by Bradford assay) |
Gene Name : | PRKAB1 protein kinase, AMP-activated, beta 1 non-catalytic subunit [ Homo sapiens ] |
Official Symbol : | PRKAB1 |
Synonyms : | PRKAB1; protein kinase, AMP-activated, beta 1 non-catalytic subunit; 5-AMP-activated protein kinase subunit beta-1; AMPK beta 1; AMPKb; AMPK beta -1 chain; AMPK subunit beta-1; AMP-activated protein kinase beta subunit; 5-AMP-activated protein kinase beta-1 subunit; protein kinase, AMP-activated, noncatalytic, beta-1; AMPK; HAMPKb; MGC17785; |
Gene ID : | 5564 |
mRNA Refseq : | NM_006253 |
Protein Refseq : | NP_006244 |
MIM : | 602740 |
UniProt ID : | Q9Y478 |
Chromosome Location : | 12q24.1-q24.3 |
Pathway : | AMPK signaling, organism-specific biosystem; Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Direct p53 effectors, organism-specific biosystem; Energy Metabolism, organism-specific biosystem; Energy dependent regulation of mTOR by LKB1-AMPK, organism-specific biosystem; Hypertrophic cardiomyopathy (HCM), organism-specific biosystem; |
Function : | AMP-activated protein kinase activity; protein binding; protein kinase binding; |
Products Types
◆ Recombinant Protein | ||
Prkab1-5110M | Recombinant Mouse Prkab1 Protein, Myc/DDK-tagged | +Inquiry |
PRKAB1-0735H | Recombinant Human PRKAB1 Protein (G2-I270), Tag Free | +Inquiry |
PRKAB1-4329R | Recombinant Rat PRKAB1 Protein, His (Fc)-Avi-tagged | +Inquiry |
PRKAB1-3419R | Recombinant Rhesus Macaque PRKAB1 Protein, His (Fc)-Avi-tagged | +Inquiry |
PRKAB1-0736H | Recombinant Human PRKAB1 Protein (G2-I270), GST tagged | +Inquiry |
◆ Lysates | ||
AMPK-416HCL | Recombinant Human AMPK cell lysate | +Inquiry |
AMPK-412HCL | Recombinant Human AMPK cell lysate | +Inquiry |
AMPK-411HCL | Recombinant Human AMPK cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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