Recombinant Human metallothionein 3, His-tagged

• Cat.No.: MT3-438H
• Product Overview: MT3, 1-68aa, Human, His tag, E.coli

Specification

• Description: Metallothionein 3, also known as MT3, binds heavy metals. This protein contains three zinc and three copper atoms per polypeptide chain and only a negligible amount of cadmium. This protein inhibits survival and neurite formation of cortical neurons in vitro. Recombinant human MT3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol
• Molecular Mass: 9.3 kDa (91aa) confirmed by MALDI-TOF(Molecular size on SDS-PAGE will appear higher)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMDPETCP CPSGGSCTCA DSCKCEGCKC TSCKKSCCSC CPAECEKCAK DCVCKGGEAA EAEAEKCSCC Q
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: MT3 metallothionein 3 [ Homo sapiens ]
• Official Symbol: MT3
• Synonyms: MT3; metallothionein 3; metallothionein 3 (growth inhibitory factor (neurotrophic)); metallothionein-3; GIF; MT-3; MT-III; metallothionein-III; growth inhibitory factor; GIFB; GRIF; ZnMT3
• Gene ID: 4504
• mRNA Refseq: NM_005954
• Protein Refseq: NP_005945
• MIM: 139255
• UniProt ID: P25713
• Chromosome Location: 16q13
• Function: antioxidant activity; cadmium ion binding; copper ion binding; copper ion binding; cysteine-type endopeptidase inhibitor activity involved in apoptotic process; drug binding; metal ion binding; protein binding; protein kinase activator activity; zinc ion