Recombinant Human platelet factor 4, His-tagged

• Cat.No.: PF4-590H
• Product Overview: CXCL4, 32-101aa, Human, His tag, E.coli

Specification

• Description: CXCL4 is a member of the CXC chemokine family. This chemokine is released from the alpha granules of activated platelets in the form of a homotetramer which has high affinity for heparin and is involved in platelet aggregation. This protein is chemotactic for numerous other cell type and also functions as an inhibitor of hematopoiesis, angiogenesis and T-cell function. Recombinant human CXCL4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT
• Molecular Mass: 10.0 kDa (91aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MEAEEDGDLQ CLCVKTTSQV RPRHITSLEV IKAGPHCPTA QLIATLKNGR KICLDLQAPL YKKIIKKLLE S
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: PF4 platelet factor 4 [ Homo sapiens ]
• Official Symbol: PF4
• Synonyms: PF4; platelet factor 4; chemokine (C X C motif) ligand 4; CXCL4; SCYB4; iroplact; oncostatin-A; C-X-C motif chemokine 4; chemokine (C-X-C motif) ligand 4; PF-4; MGC138298
• Gene ID: 5196
• mRNA Refseq: NM_002619
• Protein Refseq: NP_002610
• MIM: 173460
• UniProt ID: P02776
• Chromosome Location: 4q12-q21
• Pathway: CXCR3-mediated signaling events, organism-specific biosystem; Cell surface interactions at the vascular wall, organism-specific biosystem; Chemokine receptors bind chemokines, organism-specific biosystem; Chemokine signaling pathway, organism-specific biosystem; Chemokine signaling pathway, conserved biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Common Pathway, organism-specific biosystem
• Function: chemokine activity; heparin binding