Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Active Recombinant Human FCGR3A, His & AVI Tagged, Biotinylated, 176 Val

Cat.No. : FCGR3A-2965H
Product Overview : A DNA sequence encoding the extracellular domain (Met 1-Gln 208) of human CD16a (AAH17865.1) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin–Protein ligase (BirA enzyme) which is co-expressed.
  • Specification
  • Gene Information
  • Related Products
Description : Receptors for the Fc region of immunoglobin G (Fcγ R) are divided into three classes and CD16 (Fcγ RIII) is a multifunctional, low/intermediate affinity receptor. In human, CD16 is expressed as two distinct forms (CD16a and CD16b) encoded by two different highly homologous genes in a cell type-specific manner. CD16b is a low-affinity, GPI-linked receptor expressed by neutrophils and eosinophils, whereas CD16a is an intermediate affinity polypeptide-anchored transmembrane glycoprotein expressed by a subset of T lymphocytes, natural killer (NK) cells, monocytes, and macrophages. CD16a has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain and delivers an activation signal in the immune responses.
Source : Human Cells
Species : Human
Tag : His & AVI Tag
Form : Lyophilized from sterile PBS , pH 7.4
Bio-activity : 1. Measured by its ability to bind recombinant human IgG1 in a functional ELISA.2. Measured by its binding ability in a functional ELISA. Immobilized human CD16a-AVI-His at 10 μg/ml (100μl/well) can bind recombinant human IgG1 (Fc) with a linear range of 0.31-5 μg/ml.
Molecular Mass : The secreted recombinant human CD16a consists of 227 amino acids and predicts a molecular mass of 25.6 KDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of the protein is approximately 48 KDa due to glycosylation.
Protein length : Met1-Gln208
Endotoxin : < 1.0 eu per μg of the protein as determined by the LAL method.
Purity : >90 % as determined by SDS-PAGE
Storage : Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : Reconstitute with water to desired concentration.
Gene Name : FCGR3A Fc fragment of IgG, low affinity IIIa, receptor (CD16a) [ Homo sapiens ]
Official Symbol : FCGR3A
Synonyms : FCGR3A; Fc fragment of IgG, low affinity IIIa, receptor (CD16a); Fc fragment of IgG, low affinity IIIa, receptor for (CD16) , FCG3, FCGR3; low affinity immunoglobulin gamma Fc region receptor III-A; CD16; CD16a; FcgammaRIIIA; CD16a antigen; fc-gamma RIII; Fc-gamma RIIIa; Fc-gamma RIII-alpha; igG Fc receptor III-2; Fc gamma receptor III-A; Fc-gamma receptor IIIb (CD16); neutrophil-specific antigen NA; Fc-gamma receptor III-2 (CD 16); immunoglobulin G Fc receptor III; Fc fragment of IgG, low affinity III, receptor for (CD16); FCG3; CD16A; FCGR3; IGFR3; FCR-10; FCRIII; FCGRIII; FCRIIIA;
Gene ID : 2214
mRNA Refseq : NM_000569
Protein Refseq : NP_000560
MIM : 146740
UniProt ID : P08637
Chromosome Location : 1q23
Pathway : Adaptive Immune System, organism-specific biosystem; Fc gamma R-mediated phagocytosis, organism-specific biosystem; Fc gamma R-mediated phagocytosis, conserved biosystem; Immune System, organism-specific biosystem; Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell, organism-specific biosystem; Leishmaniasis, organism-specific biosystem; Leishmaniasis, conserved biosystem;
Function : IgG binding; receptor activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (7)

Ask a question
How does FCGR3A mediate immune cell activation and phagocytosis in response to antibody-opsonized targets? 07/29/2022

FCGR3A activates immune cells and mediates phagocytosis, aiding in the clearance of pathogens and antibody-coated targets.

What role does FCGR3A play in antibody-dependent cellular cytotoxicity, particularly in cancer immunotherapy? 03/03/2020

FCGR3A is key in antibody-dependent cellular cytotoxicity, crucial for the effectiveness of certain cancer immunotherapies.

How does genetic variation in the FCGR3A gene affect individual responses to monoclonal antibody treatments? 12/29/2018

Genetic variations in FCGR3A influence responses to monoclonal antibody treatments, affecting treatment efficacy.

What are the mechanisms by which FCGR3A regulates the balance between immune activation and tolerance? 12/06/2018

FCGR3A helps regulate the immune system's balance between activation and tolerance, preventing overactive immune responses.

How do alterations in FCGR3A expression or function affect the efficacy of vaccines? 08/12/2018

Changes in FCGR3A function or expression can influence vaccine efficacy, impacting immune system responses to vaccination.

How does FCGR3A contribute to the inflammatory response in infectious diseases? 01/25/2018

FCGR3A plays a role in the inflammatory response to infections, contributing to pathogen clearance and immune regulation.

What is the impact of FCGR3A expression on the development and severity of autoimmune diseases? 01/08/2018

FCGR3A expression impacts the development and severity of autoimmune diseases by modulating immune responses.

Customer Reviews (3)

Write a review
Reviews
12/28/2021

    Outstanding protein-protein interaction studies, boosts our experiments.

    02/04/2021

      Swift protein quantification, improves data reliability.

      11/21/2019

        Expertise in peptide synthesis, accelerates our peptide-based projects.

        Ask a Question for All FCGR3A Products

        Required fields are marked with *

        My Review for All FCGR3A Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends