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Active Recombinant Mouse Fcgr2b protein(Met1-Arg217), His&Avi-tagged, Biotinylated

Cat.No. : Fcgr2b-2968M
Product Overview : Biotinylated Recombinant Mouse FGGR2B (NP_001070657.1)(Met 1-Arg 217) was expressed in HEK293, fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus.
  • Specification
  • Gene Information
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Source : HEK293
Species : Mouse
Tag : C-His&Avi
Protein length : Met1-Arg217
Conjugation/Label : Biotin
Form : Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity : 1.Measured by its ability to bind human IgG1 in a functional ELISA. 2. Loaded Biotinylated Mouse FcγRIIB / CD32b recombinant protein (His & Avi Tag) on SA Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 0.04 μM as determined in a BLI assay (ForteBio Octet Red384).
Molecular Mass : The secreted recombinant mouse FGGR2B consists of 212 amino acids and has a predicted molecular mass of 23.9 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm FGGR2B is approximately 38-43 kDa due to glycosylation.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : Fcgr2b Fc receptor, IgG, low affinity IIb [ Mus musculus ]
Official Symbol : Fcgr2b
Synonyms : FCGR2B; Fc receptor, IgG, low affinity IIb; low affinity immunoglobulin gamma Fc region receptor II-b; fcRII; FcgammaRIIB; Fc-gamma-RII; fc-gamma RII; Fc gamma RIIB; fc-gamma-RIIB; fc gamma receptor IIB; lymphocyte antigen 17; igG Fc receptor II beta; low affinity immunoglobulin gamma Fc region receptor II; CD32; Fcgr2; Fcr-2; Fcr-3; Ly-17; LyM-1; Lym-1; FcgRII; Fcgr2a; Ly-m20; AI528646; Fc[g]RII; F630109E10Rik;
Gene ID : 14130
mRNA Refseq : NM_001077189
Protein Refseq : NP_001070657

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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