Recombinant full length Human Cu (I)-ATOX1 (aa 1-68) was expressed in E. coli. Binding of Cu (I) to the protein was confirmed by ICP.
This gene encodes a copper chaperone that plays a role in copper homeostasis by binding and transporting cytosolic copper to ATPase proteins in the trans-Golgi network for later incorporation to the ceruloplasmin. This protein also functions as an antioxidant against superoxide and hydrogen peroxide, and therefore, may play a significant role in cancer carcinogenesis. Because of its cytogenetic location, this gene represents a candidate gene for 5q-syndrome.
In 20 mM Phosphate buffer pH 7.0.
MPKHEFSVDM TCGGCAEAVS RVLNKLGGVK YDIDLPNKKV CIESEHSMDT LIATLKKTGK TVSYLGLE
>95% by SDS-PAGE. The protein was observed as a single band migrating at a molecular weight < 10 kDa.
To avoid oxidation and leaking of the Cu(I) bound to the protein, keep and use the sample under inert atomosphere.
Store at - 20 centigrade. The protein is stable at 4 centigrade for at least 2 weeks and at 25 centigrade for at least several hours. After initial deforst, aliquot product into individual tubes under inert atomosphere and refreeze at - 20 centigrade. Avoid repeated freeze/defrost cycles. Avoid exposition to air.