Recombinant Human ATOX1 Protein, with Cu (I)

• Cat.No.: ATOX1-225H
• Product Overview: Recombinant full length Human Cu (I)-ATOX1 (aa 1-68) was expressed in E. coli. Binding of Cu (I) to the protein was confirmed by ICP.

Specification

• Description: This gene encodes a copper chaperone that plays a role in copper homeostasis by binding and transporting cytosolic copper to ATPase proteins in the trans-Golgi network for later incorporation to the ceruloplasmin. This protein also functions as an antioxidant against superoxide and hydrogen peroxide, and therefore, may play a significant role in cancer carcinogenesis. Because of its cytogenetic location, this gene represents a candidate gene for 5q-syndrome.
• Source: E. coli
• Species: Human
• Form: In 20 mM Phosphate buffer pH 7.0.
• Molecular Mass: 7.4 kDa
• Protein length: aa 1-68
• AA Sequence: MPKHEFSVDM TCGGCAEAVS RVLNKLGGVK YDIDLPNKKV CIESEHSMDT LIATLKKTGK TVSYLGLE
• Purity: >95% by SDS-PAGE. The protein was observed as a single band migrating at a molecular weight < 10 kDa.
• Notes: To avoid oxidation and leaking of the Cu(I) bound to the protein, keep and use the sample under inert atomosphere.
• Storage: Store at - 20 centigrade. The protein is stable at 4 centigrade for at least 2 weeks and at 25 centigrade for at least several hours. After initial deforst, aliquot product into individual tubes under inert atomosphere and refreeze at - 20 centigrade. Avoid repeated freeze/defrost cycles. Avoid exposition to air.
• Concentration: 1 mg/ml

Gene Information

• Gene Name: ATOX1 ATX1 antioxidant protein 1 homolog (yeast) [ Homo sapiens ]
• Official Symbol: ATOX1
• Synonyms: ATOX1; ATX1 antioxidant protein 1 homolog (yeast); ATX1 (antioxidant protein 1, yeast) homolog 1; copper transport protein ATOX1; HAH1; metal transport protein ATX1; ATX1; MGC138453; MGC138455
• Gene ID: 475
• mRNA Refseq: NM_004045
• Protein Refseq: NP_004036
• MIM: 602270
• UniProt ID: O00244
• Chromosome Location: 5q32
• Pathway: Mineral absorption, organism-specific biosystem; Mineral absorption, conserved biosystem
• Function: copper chaperone activity; copper ion binding; copper-dependent protein binding; metal ion binding; metallochaperone activity