Recombinant Human BTLA(Lys 31 - Ser 150 ) fused with a human IgG1 Fc tag at C-terminal was expressed in HEK293.
Predicted N Terminal:
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally Trehalose is added as protectant before lyophilization.
Has a calculated MW of 40.4 kDa. The reducing (R) protein migrates as 50-60 kDa in SDS-PAGE due to glycosylation.
Lys 31 - Ser 150
Less than 1.0 EU per μg by the LAL method.
>92% as determined by SDS-PAGE.
Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).