Active Recombinant H1N1 HA, His-tagged

• Cat.No.: HA-420H
• Product Overview: A DNA sequence encoding the Influenza A virus (A/USSR/90/1977(H1N1)) hemagglutinin (P03453.2) (Met1-Gln529) was expressed with a C-terminal polyhistidine tag.

Specification

• Source: Baculovirus-Insect cells
• Species: H1N1
• Tag: His
• Form: Lyophilized from sterile PBS, pH7.4.
• Bio-activity: 1. Measured by its ability to bind with Neu5Aca2-3Galb1-4GlcNAcb-PAA-biotin (01-077) using the Octet RED System.2. Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 0.01-0.04 μg/mL for 1% GRBC.
• Molecular Mass: The recombinant hemagglutinin of Influenza A virus (A/USSR/90/1977(H1N1))comprises 523 amino acids and has a predicted molecular mass of 59.4 kDa. The apparent molecular mass of the protein is approximately 65.3 kDa in SDS-PAGE under reducing conditions.
• Protein length: Met1-Gln529
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: Hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Gene Name: HA?haemagglutinin [?Influenza A virus (A/Puerto Rico/8/1934(H1N1))?]
• Official Symbol: HA
• Synonyms: HA; haemagglutinin; HA1; HA2
• Gene ID: 956529
• Protein Refseq: NP_040980
• Chromosome Location: segment: segment 4
• Pathway: Assembly of Viral Components at the Budding Site; Entry of Influenza Virion into Host Cell via Endocytosis; Fusion and Uncoating of the Influenza Virion