Active Recombinant Mouse Epha3 protein(Met1-His541), His-tagged

• Cat.No.: Epha3-495M
• Product Overview: Recombinant Mouse EPHA3 (NP_034270.1) (Met1-His541) was expressed in HEK293 with a C-terminal polyhistidine tag.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-His
• Protein length: Met1-His541
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. 2. Immobilized mouse EPHA3-His at 10 μg/mL (100 μL/well) can bind mouse EFNA5-Fc. The EC50 of mouse EFNA5-Fc is 4.9-11.4ng/mL.
• Molecular Mass: The recombinant mouse EPHA3 comprises 532 amino acids and has a predicted molecular mass of 60.2 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Epha3 Eph receptor A3 [ Mus musculus ]
• Official Symbol: Epha3
• Synonyms: EPHA3; Eph receptor A3; ephrin type-A receptor 3; EK4; EPH-like kinase 4; tyrosine-protein kinase TYRO4; tyrosine-protein kinase receptor ETK1; Hek; Cek4; ETK1; End3; Hek4; Mek4; Tyro4; AW492086
• Gene ID: 13837
• mRNA Refseq: NM_010140
• Protein Refseq: NP_034270