Recombinant Human TYK2, His-tagged

• Cat.No.: TYK2-702H
• Product Overview: Recombinant Human TYK2(a.a. 871-1187), fused with N-terminal His-tag, was expressed in a Baculovirus-infected Sf9 cell expression system.

Specification

• Description: This gene encodes a member of the tyrosine kinase and, more specifically, the Janus kinases (JAKs) protein families. This protein associates with the cytoplasmic domain of type I and type II cytokine receptors and promulgate cytokine signals by phosphorylating receptor subunits. It is also component of both the type I and type III interferon signaling pathways. As such, it may play a role in anti-viral immunity. A mutation in this gene has been associated with hyperimmunoglobulin E syndrome (HIES) - a primary immunodeficiency characterized by elevated serum immunoglobulin E.
• Source: Baculovirus-infected Sf9 cell
• Species: Human
• Tag: His
• Form: 45 mM Tris, pH 8, 125 mM NaCl, 2.4 mM KCl, 0.045% Tween-20, 100 mM imidazole and 10% glycerol.
• Molecular Mass: 38 kDa
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Quality Control Test: Assay conditions: Assay was done in Kinase buffer containing 0.2 mM DTT using Poly-(Glu4:Tyr)-biotin substrate (0.2 mg/ml) and 20 mM ATP. Reaction was done at 30°C for 30 minutes. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).
• Stability: >6 months at –80°C

Gene Information

• Gene Name: TYK2 tyrosine kinase 2 [ Homo sapiens (human) ]
• Official Symbol: TYK2
• Synonyms: TYK2; JTK1; tyrosine kinase 2; non-receptor tyrosine-protein kinase TYK2; NP_003322.3; EC 2.7.10.2
• Gene ID: 7297
• mRNA Refseq: NM_003331
• Protein Refseq: NP_003322
• MIM: 176941
• UniProt ID: P29597
• Chromosome Location: 19p13.2
• Pathway: Cytokine Signaling in Immune system; Epstein-Barr virus infection; Herpes simplex infection
• Function: ATP binding; growth hormone receptor binding; non-membrane spanning protein tyrosine kinase activity