| Description : |
Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in non-muscle cells. Mutations in this gene are associated with DFNA20/26, a subtype of autosomal dominant non-syndromic sensorineural progressive hearing loss. Alternative splicing results in multiple transcript variants. |
| Source : |
E. Coli |
| Species : |
Human |
| Tag : |
His,GST |
| Form : |
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl. |
| Molecular Mass : |
73.8kDa |
| Protein length : |
375 |
| Endotoxin : |
<1.0eu per 1ug (determined by the lal</1.0eu |
| Purity : |
>95% |
| Applications : |
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP) |
| Notes : |
Small volumes of ACTg1 recombinant protein may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice. |
| Storage : |
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition. |
| Reconstitution : |
Reconstitute in sterile PBS, pH7.2-pH7.4. |
| Warning : |
This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
