Recombinant Rat ALT, His-tagged

• Cat.No.: ALT-3248R
• Product Overview: Alanine Aminotransferase (ALT)

Specification

• Description: human homolog is a cytosolic alanine aminotransferase .
• Source: E. Coli
• Species: Rat
• Tag: His
• Form: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Molecular Mass: 56.6kDa
• Protein length: 496
• Endotoxin: <1.0eu per 1ug (determined by the lal</1.0eu
• Purity: >95%
• Applications: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Notes: Small volumes of ALT recombinant protein may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Warning: This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Gene Information

• Gene Name: Gpt glutamic-pyruvate transaminase (alanine aminotransferase) [ Rattus norvegicus ]
• Official Symbol: Gpt
• Synonyms: Gpt1; alanine aminotransferase 1; ALT1; GPT 1; glutamic pyruvate transaminase; glutamic--alanine transaminase 1; glutamic--pyruvic transaminase 1; glutamate pyruvate transaminase 1; glutamic pyruvic transaminase 1, soluble; glutamic pyruvic transaminase (alanine aminotrasferase)
• Gene ID: 81670
• mRNA Refseq: NM_031039
• Protein Refseq: NP_112301
• UniProt ID: P25409
• Chromosome Location: 7q34
• Pathway: 2-Oxocarboxylic acid metabolism, organism-specific biosystem; Amino acid synthesis and interconversion (transamination), organism-specific biosystem
• Function: L-alanine:2-oxoglutarate aminotransferase activity; pyridoxal phosphate binding