Active Native Bacillus stearothermophilus Acetate Kinase
Cat.No. : | Acetate Kinase-22B |
Product Overview : | Native Bacillus stearothermophilus Acetate Kinase was purified from Bacillus stearothermophilus. |
- Specification
- Gene Information
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Description : | Acetate kinase plays an important role in glycolysis. Acetate kinase phosphorylates acetate in the presence of ATP and a divalent cation, which ultimately results in the production of acetyl-CoA. Acetate kinase is also involved in the metabolism of propanoate, pyruvate and taurine. Acetate Kinase from Bacillus stearothermophilus is a thermostable tetramer of identical subunits with molecular weight of 40,000 Da each. The enzyme does not have a -SH group and is composed of 36% β-structure, 21 α-helix and 43 % unordered structure. |
Source : | Bacillus stearothermophilus |
Species : | Bacillus stearothermophilus |
Form : | Lyophilized powder containing potassium phosphate. |
Bio-activity : | Involved in the metabolism of propanoate, pyruvate and taurine. 400-1,200 units/mg solid. |
Unit Definition : | One unit will phosphorylate 1.0 μmole of acetate to acetyl phosphate per min at pH 7.2 at 30 °C. |
Applications : | Acetate kinase is used to phosphorylate acetate to acetyl phosphate. Acetate Kinase from Bacillus stearothermophilus has been used to study allosteric activation. [32P]-acetyl phosphate was generated by incubating potassium acetate in the reaction mixture with acetate kinase from Sigma. This [32P]-acetyl phosphate was used to label BldM, BldM D-54N or BldM D-54A loci during the study of the effect of bldM gene on Streptomyces coelicolor development. |
Storage : | 2-8°C. |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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